Specifications

Fluorometer

General Design

Signal detection: PIN-photodiode protected by long-pass filter (T(50%)=715 nm). Selective window amplifier

Sockets: Connector for Special Fiberoptics 2010-F. USB socket. Sockets for Leaf Clip Holder 2030-B or Micro Quantum/Temperature-Sensor 2060-M, Battery Charger MINI-PAM/L or external 12 V battery via MINIPAM/AK cable, and external lamp/trigger output

Communication: USB. Bluetooth Version 2.0 + EDR Class 2

User interface: Windows computer with PamWin-3 software

Power consumption: Basic operation 1.6 W, 8 W with all internal light sources operated at maximum output (measuring light, red and blue actinic light, and far red light). Saturation pulse at maximum intensity, 30 W

Power supply: Rechargeable sealed lead-acid battery 12 V/2 Ah; Battery Charger MINI-PAM/L (100 to 240 V AC)

Recharging time: 6 hours (with the PAM-2500 turned off) via Battery Charger MINI-PAM/L

Operating temperature:
-5 to +40 °C

Operating humidity range: 20 to 95% RH to avoid condensation

Dimensions: 23 cm x 10.5 cm x 10.5 cm (L x W x H) aluminum housing

Weight: 2.5 kg (including battery)

Light Output | PAM-2500

Measuring light: Red LEDs, maximum emission at 630 nm, FWHM (full width at half maximum) 20 nm. 1 μs pulses at modulation frequencies 10 to 5000 Hz for Fo determinations (200 Hz default), and 1 to 100 kHz during actinic illumination, fast kinetics with 100 or 200 kHz, 20 intensity levels, frequency dependent effective PAR ranging from 0.001 to 100 μmol m-2 s-1

Blue actinic light: LEDs, maximum emission at 455 nm, FWHM 20 nm, PAR up to 800 μmol m-2 s-1, 20 intensity levels

Red actinic light: LEDs, maximum emission at 630 nm, FWHM 15 nm, PAR up to 4000 μmol m-2 s-1, 20 intensity levels

Saturation pulses: Red LEDs (see red actinic light), PAR up to 25000 μmol m-2 s-1, adjustable between 0.1 and 0.8 s, 20 intensity levels

Multiple turn-over flashes: Red LEDs (see red actinic light), PAR up to 25000 μmol m-2 s-1, adjustable between 1 and 800 ms, 20 intensity levels

Single turn-over flashes: Red LEDs (see red actinic light), PAR up to 125000 μmol m-2 s-1, adjustable between 5 and 50 μs

Far-red light: LED, maximum emission at 750 nm, FWHM 25 nm, 20 intensity levels

Special Fiberoptics
2010-F

Design: Flexible, steel-spiral, plastic-covered bundle with three-pin optical connector

Joint end (measuring site): Active diameter 6 mm, outer diameter 8 mm

Length: 100 cm

Weight: 300 g

Distance Clip 60° 2010-A

Design: Metal clip with fiber holder and 11 mm sample hole: 5.5 cm x 1.4 cm (L x W)

Fiber holder: 1.2 cm length, mounted 0.7 cm above base, with lateral screw to fix fiber optics. Angle between fiber optics axis and sample plane: 60°. Two spacer rings to vary the distance between fiber end and leaf surface

External Voltage Supply Cable MINI-PAM/AK

Design: 90 cm cable with fuse for over-current protection. The cable bypasses the internal battery of the PAM-2500 fluorometer and supplies voltage from an external 12 V battery directly to the electronics.

Carrier Bag

Carrier: Robust field carrier bag with shoulder and hip belt

System Control and Data Acquisition

Software: PamWin-3 System Control and Data Acquisition Program

Fluorescence parameters:

Measured: Ft, Fo, Fm, F, Fo' (also calculated), Fm'. Fast polyphasic rise and decay kinetics (time resolution up to 10 µs). PAR and °C, using the “Leaf-Clip Holder 2030-B” or the “Micro Quantum/Temp.-Sensor 2060-M”)

Calculated: Fo' (also measured), Fv/Fm and Y(II) (maximum and effective photochemical yield of PS II, respectively), qL, qP, qN, NPQ, Y(NPQ), Y(NO) and ETR (electron transport rate), C/Fo (constant fraction of Fo that is assumed to originate from PS I)

Computer Requirements

Processor, 0.8 GHz. RAM, 512 MB. Hard disc space, 50 MB. Screen resolution, 1024 x 600 pixels. Interface, USB 1.1 or USB 2.0. Optional interface, Bluetooth Version 2.0 + EDR, Widcomm Stack. Operating system: Microsoft Windows XP SP2/Vista

Samsung Q1 Ultra PC UMPC-1

Ultra-mobile touchscreen computer SAMSUNG Q1 Ultra

Hardware: Processor, Intel A110 800MHz ULV. Cache, L2, 512 KByte. Bus speed, 400 MHz. DDR II SDRAM, 1024MB. Hard disc, 60 GByte, 4200 rpm. Display, 7" WSVGA Touchscreen Glare TFT (1024x600)

Interface: 2 x USB 2.0, LAN, 802.11b/g WLAN, Bluetooth 2.0 EDR. Card reader, SD, MMC. Integrated webcams, front 0.3 megapixel, back 1.3 megapixel

Graphics: Intel GMA950 controller onboard, maximum 128 MByte shared memory

Operating system: Windows XP Tablet PC Edition

Battery: 2 lithium-ion batteries, 7.4 V DC 4.0 Ah and 7.4 V DC 7.8 Ah (up to 3.5 and 6 hours, respectively, of battery life). Power supply, 100-240 V AC, 50-60 Hz

Dimensions: 22.8 cm x 12.4 cm x 2.4 cm (L x W x H)

Weight: 0.7 kg

Computer box: Aluminum compartment, 23.5 cm x 15.0 cm x 4.5 cm (L x W x H), on top of PAM-2500 chassis to keep an ultra-mobile touchscreen PC

Leaf-Clip Holder 2030-B

Micro quantum sensor: Selective PAR measurement, 0 to 20000 μmol m-2 s-1 PAR

Thermocouple: Ni-CrNi, diameter 0.1 mm, -20 to +60 °C

Output: PAR, high sensitivity range: 0 to 1000 μmol m-2 s-1; normal sensitivity range: 0 to 20000 μmol m-2 s-1 (output 0 to 2.5 V for each range). Leaf temperature, -20 to +60 °C (0 to 0.8 V). Remote trigger button

Power supply: PAM-2500 leaf clip socket (5 V/4 mA)

Cable length: 100 cm

Dimensions: 17 cm x 5.7 cm (max.) x 8 cm (max.) (L x W x H)

Weight: 310 g

Arabidopsis Leaf Clip
2060-B

Design: Aluminum clip, prepared to accommodate PAR and temperature sensors of Micro Quantum/Temperature-Sensor 2060

Dimensions: 7.6 cm x 4.9 cm (max.) x 5.2 cm (max.) (L x W x H)

Weight: 65 g

Arabidopsis Leaf Holder DUAL-BA

Design: Tube-shaped fiber tip holder composed of polyoxymethylene (POM; 4.5 cm x 2.5 cm, L x D max,) with laterally recessed permanent samarium-cobalt magnet. Spring steel band (0.3 mm thick, 1.5 cm wide) consisting of two sides (2 cm and 5 cm, respectively) which form a right angle. The leaf is positioned between the 2 cm side of the steel band and the fiber optics tip. The steel band is attached to the fiber tip holder by placing the 5 cm side of the steel band on the fiber tip holder’s magnet. Including a guide loop (length 3 cm, diameter 2.5 cm) for fiber optics with an 11 cm steel rod (0.8 cm diameter) for mounting to a stand

Dark Leaf Clip DLC-8

Design: Clip made of aluminum with felt contact areas and sliding shutter

Dimensions: 6.5 cm x 2 cm (max.) x 1.5 cm (max.) (L x W x H)

Weight: 3.6 g

External Battery 000160101314

Battery: 12 V battery, 2.1 Ah, 0.84 kg

Dimensions: 3.4 cm x 17.8 cm x 6.0 cm. Charge cycle, 2 hours