DUAL-KLAS-NIR

Version:

Product

P700, Plastocyanin, Ferredoxin & Chlorophyll Fluorescence Measuring System

DUAL-KLAS-NIR

General design
Microcontroller: 4 x AVR-RISC (8 MHz) + 4 MB SRAM; 256 000 data points with 12 bit resolution can be stored
Communication
PC interface: USB 1.1, 2.0 and 3.0 compatible
User interface

Windows computer (Windows 7/8/10/11) with KLAS-100 software

Power supply
Rechargeable sealed lead-acid battery 12 V/2 Ah; Battery Charger MINI-PAM/L (100 to 240 V AC)
Power consumption
During basic operation 350 mA
Sockets
6 ports for heads (power for single wavelength (2 channels) and double wavelength (4 channels) modulated measuring light, power for 2 actinic LED arrays (one in DKN-E and one in DKN-D) and 1 NIR measuring light LED array (in DKN-E), input for 2 detectors (the photodiode detector of DKN-D is connected to detector 1), socket for stirrer (plus speed controller and standby switch), AUX (for Spherical Micro Quantum Sensor US-SQS/WB or Cosine-Corrected Mini Quantum Sensor US-MQS/WB), USB (for USB cable), TRIGGER OUT (output of 5 V rectangular signals to trigger external devices), 2 EXT. SIGNALS (input for external DC signals. Range 0-1 V or 0-5 V), and CHARGE (for MINI-PAM/L charger).
Dimensions
36 cm x 16 cm x 26.5 cm (W x H x D), with carrying handle
Weight
5.8 kg
Measuring light
Chip-On-Board LED array for pulse-modulated 780, 820, 840, 870 and 965 nm NIR measuring light, pulse modulated green, fluorescence measuring light (540 nm).
Actinic light
Chip-On-Board LED array (635 nm) for continuous red actinic illumination (max. ~3400 µmol photons m-2 s-1 PAR), which can also be used for single turn over flashes (max. >28000 µmol photons m-2 s-1 PAR, length 5-50 µs), and multiple turnover pulses (MT and SP) (max. 25000 µmol photons m-2 s-1 PAR, length 1-1000 ms).
Dimensions
5.5 cm x 9 cm x 4 cm (W x H x D)
Weight
560 g (incl. cables, 1.4 m long)
Measuring light
pulse modulated blue fluorescence measuring light (460 nm).
Actinic light
Blue light (460 nm) for continuous blue illumination (max. ~400 µmol m-2 s-1 PAR), Chip-On-Board LED array (635 nm) for continuous red illumination (max. ~3400 µmol m-2 s-1 PAR), which can also be used for saturating single turnover flashes (max. >28000 µmol m-2 s-1 PAR, length 5-50 µs), and multiple turnover pulses (MT and SP) (max. 25000 µmol photons m-2 s-1 PAR, length 1-1000 ms), far-red light (740 nm) for continuous far-red illumination (max. 400 µmol photons m-2 s-1).
Signal detection
Photodiode and pulse preamplifier to measure four dual wavelength NIR-transmission difference signals (780-820, 820-870, 870-965 and 840-965 nm) and two fluorescence signals. Time resolution of a single channel measurement is 35 µs, of a two channel measurement is 150 µs and of a 6 channel measurement is 1 ms. The same detector (protected by the same set of filters) is used for fluorescence and NIR transmission measurements. The filter set passes wavelengths > 750 nm.
Dimensions
5.5 cm x 9 cm x 4 cm (W x H x D)
Weight
540 g (including cables, 1.4 m long)
Design
Aluminum box with custom foam packing for DUAL-KLAS-NIR and accessories
Dimensions
60 cm x 40 cm x 34 cm (L x W x H)
Weight
5 kg
Design
Consisting of black anodized aluminum baseplate with gear rack on which one measuring head holder is mounted on a movable stage which can be precisely positioned along the gear rack by a lateral adjustment knob. Includes a 13 cm lab stand rod, which can be fixed (screwed) to the bottom of the baseplate, and a 3 mm Allen wrench
Dimensions
18.5 cm x 11.5 cm x 12 cm (L x W x H, max. without lab stand rod)
Weight
1050 g
Achievable temperatures
12 K below ambient temperature, 15 K above ambient temperature (Quartz cuvette placed in Optical Unit for Suspensions ED-101US/MD with 1.5 mL water and stirrer PHYTO-MS on)
Size
ø 55 mm, 110 mm height
Cable length
130 cm
Weight
0.29 kg (including cable)

Accessories

Design
Cuvette is a sandwich of two 2 x 2 cm aluminum frames, each holding the end part of a Walz standard Perspex rod to connect various measuring heads of the DUAL-PAM-100 or DUAL-KLAS-NIR. Distance between Perspex rod and leaf: ca. 1 mm on each leaf side.
Pneumatically separated upper and lower cuvette halves. Controlled by a regulator unit with sockets for cable connections to the Control Unit 3000 C of the GFS-3000 and a trigger input line.
Cuvette temperature
Pt 100 type A (located near the Peltier elements), range -10 to 50 °C, accuracy ±0.1 °C
Temperature control
Set point value for cuvette temperature. Cuvette temperature ranging from 10 degrees below ambient to max. +50 °C
Leaf temperature measurement
Thermocouple, range -10 to 50 °C, accuracy ±0.2 °C
External miniature quantum sensor
Mini Quantum Sensor MQS-B/GFS: Selective PAR measurement, range 0 to 2500 μmol m-2 s-1 PAR, accuracy ±5%, cosine corrected
Leaf area
1.3 cm2
Trigger in
Triggers at 5 V → 0 V signal change
Operating temperature
-5 to 45 °C
Dimensions
Assembled cuvette: 10 cm x 4 cm x 12 cm (L x W x H), electronics box : 7 cm x 7 cm x 15 cm (L x W x H)
Weight
Cuvette, electronics box, cables, and mounting frame: 1.7 kg; Mounting Stand ST-101: 2 kg
Design

Black-anodized aluminum body with central 10 x 10 mm glass cuvette; for attachment of Measuring Heads and Miniature Magnetic Stirrer PHYTO-MS; additional ports for attachment of two additional measuring heads

Weight

750 g

Temperature Control Block ED-101US/T
Sectioned block with central 10 x 10 mm opening to be mounted on top of the ED-101US/MD unit; to be connected to external flow-through water bath (not included), weight: 250 g
Miniature Magnetic Stirrer PHYTO-MS

Based on a device manufactures by h+p (type Variomag-Mini); featuring adapter to be mounted in the bottom port of the Optical Unit ED-101US/MD; powered and controlled by the Power-and-Control-Unit

Spherical Micro Quantum Sensor US-SQS/WB

3.7 mm Ø diffusing sphere coupled to integrated PAR sensor via 2 mm diameter fiber; compact amplifier unit and special holder for mounting on Optical Unit ED-101US/MD; to be connected tot the Power-and-Control Unit

Temperature Control Block ED-101US/T
Sectioned block with central 10 x 10 mm opening to be mounted on top of the ED-101US/MD unit; to be connected to external flow-through water bath (not included), weight 250 g
Miniature Magnetic Stirrer PHYTO-MS
Based on device manufactured by h+p (type Variomag-Mini); featuring adapter to be mounted in bottom port of the Optical Unit ED-101US/MD; powered and controlled by the Power-and-Control-Unit DKN-C
Spherical Micro Quantum Sensor US-SQS/WB
3.7 mm ø diffusing sphere coupled to integrated PAR sensor via 2 mm diameter fiber; compact amplifier unit and special holder for mounting on Optical Unit ED-101US/MD; to be connected to the Power-and-Control Unit DKN-C
Design
Quartz glass cuvette, cross section: 10 mm x 10 mm, external dimensions: 12.5 mm x 12.5 mm x 26 mm (L x W x H). Special cuvette holder to position the cuvette between two measuring heads. U-shaped black-anodized aluminum shields to screen out external light. Three sealing gaskets to protect lower measuring head from spills
Design

Magnetic stirrer driven by a rotating magnetic field; the PHYTO-MS is connected to Power-and-Control Unit PHYTO-II-C; a special adapter plug allows the insertion in the bottom port of the Optical Unit ED-101US/MP

Weight
16 g
Display
Three line LCD display
Control range
0 °C to 50 °C at 0.1 K steps
Operating voltage
11 V – 14 V DC
Maximum Peltier current
1 A
Size
105 mm x 90 mm x 130 mm (W x H x D)
Weight
0.57 kg

Power-and-Control Unit US-T/DR

Display
Three line LCD display
Control range
0 °C to 50 °C at 0.1 K steps
Operating voltage
11 V - 14 V DC
Maximum Peltier current
1 A
Size
105 mm x 90 mm x 130 mm (W x H x D)
Weight
0.57 kg

Peltier Heat-Transfer Head US-T/DS

Achievable temperatures
12 K below ambient temperature, 15 K above ambient temperature (Quartz cuvette placed in Optical Unit for Suspensions ED-101US/MD with 1.5 mL water and stirrer PHYTO-MS on)
Size
⌀ 55 mm, 110 mm height
Cable length
130 cm
Weight

0.29 kg (including cable)

AC Adapter

Input

100 V - 240 V AC 1.5 A 50-60 Hz

Output
12 V DC 5.5 A
Size

130 mm x 56 mm x 30 mm (L x W x H)

Weight
0.50 kg (including cable)
Dimensions

Base plate, 40 cm x 30 cm

Height

73.5 cm, diameter 1.5 cm

Weight

2.8 kg

Design
Cosine-response mini quantum sensor for selective measurement of photosynthetically active radiation (PAR, 400 – 700 nm) with Perspex diffuser disk as light entrance and signal amplifier box
Signal detection
High stability silicone photovoltaic detector with filter set providing equal response to photon fluxes across the PAR spectral range. The typical signal output of the detector is 2 μA / (1000 μmol m‑2 s‑1)
Temperature coefficient of photodiode
0.01 %/K
Absolute calibration
± 5 %
Angular dependence
error < 4 % between angles from -80° to +80° from normal axis
Immersion coefficient
Typically 1.32
Cable length
3 m
Size: Height
16 mm; diameter: 14 mm; Diffuser diameter: 5.5 mm
Weight
32 g
Amplifier box
Two amplification ranges (0 to 1000 and 0 to 20,000 μmol m‑2 s‑1, each range corresponding to 0 to 2.5 V DC). To be connected to the Leaf Clip Holder 2030-B port of the PAM-CONTROL unit. Power provided by the PAM-CONTOL unit. Dimensions: 5 cm x 3 cm x 5 (W x H x D). Weight: 200 g.