JUNIOR-PAM

Version:

Product

Teaching Chlorophyll Fluorometer

JUNIOR-PAM

Program

WinControl-3 System Control and Data Acquisition Program (Microsoft Windows 10 and 11) for operation of measuring system via PC, data acquisition and analysis. Not compatible with Windows 10 on ARM

Saturation Pulse Analysis

Measured: Ft, F0, FM, F, F0’ (also calculated), FM’. Depending on the leaf clip connected, the software can record PAR, temperature and also humidity. [In the case of the MINI-PAM-II clip humidity can be measured, which the clip of the JUNIOR-PAM cannot.]
Calculated: F0’ (also measured), FV/FM and Y(II) (maximum and effective photochemical yield of PS II, respectively), qL, qP, qN, NPQ, Y(NPQ), Y(NO) and ETR (electron transport rate)

Fitting Routines

Two routines for determination of the cardinal points α, Ik and ETRmax of light curves

Programmed Features

Automatic determination of signal offset for all light intensities and gain levels. Automatic calibration of internal PAR sensor against an external PAR sensor connected to the instrument

Computer Requirements

Processor: 0.8 GHz, RAM: 512 MB, screen resolution: 1024 x 600 pixels, interface: USB 2.0/3.0

Communication Protocol

USB

Modulated fluorescence excitation
Blue LED (wavelength of maximum emission: 445 nm). Modulation frequencies 5 to 25 Hz adjustable in increments of 5 Hz, and 100 Hz. Fluorescence at wavelengths greater than 630 nm is measured
Actinic light
Same LED as used for modulated light. Actinic PAR, 25 to 1500 μmol μmol m-2 s-1; maximum PAR of saturation pulses, 7000 μmol m-2 s-1. All data valid for 1 mm distance from the tip of the 50 cm JUNIOR-PAM light guide
Modulated fluorescence excitation
White LED (wavelengths of maximum emission: 445 and 545 nm). Modulation frequencies 5 to 25 Hz adjustable in increments of 5 Hz, and 100 Hz. Fluorescence at wavelengths greater than 650 nm is measured
Actinic light
Same LED as used for modulated light. PAR at 1 mm distance from the tip of the 50 cm JUNIOR-PAM light guide: between 25 and 1500 μmol m-2 s-1. Maximum PAR of saturation pulses, 7000 µmol m-2 s-1
Housing
Aluminum housing with USB type B socket, M8 4-pole socket to connect the JUNIOR-PAM monitoring leaf clip (accessory), port for JUNIOR-PAM light guide, and swivel-mounted sample support
Far-red light
LED with 745 nm maximum emission wavelength for selective excitation of photosystem I
Fluorescence detection
PIN photodiode protected by long-pass filter. Selective window amplifier to measure pulse-amplitude modulated (PAM) fluorescence
Communication
Standard USB communication via 1.2 m USB-cable type A-B
Power supply
5 V DC supplied by the USB-cable type A-B used for communication
Power consumption
100 mW at normal operation and 500 mW during saturation pulse
Dimensions
11.5 cm x 6.5 cm x 3 cm (L x W x H)
Weight
200 g
Operating temperature
0 to + 40 °C
Operating humidity range
35 to 85% RH (to avoid condensation)
Light guide
50 cm x 1.5 mm (length x diameter) plastic fiber
Sample clips
Open leaf clip (angle between incident radiation from JUNIOR-PAM and leaf surface, 60°) and magnetic leaf clip (angle between incident radiation from JUNIOR-PAM and leaf surface, 90°)
Fluorescence standard
3 cm x 2 cm fluorescence foil
Polishing set
Set for fiber tips including various polishing pads
USB cable
A-B USB cable
Software on USB memory key
Including latest WinControl-3 software
Transport Case JUNIOR-T
Grey plastic box with handle. Interior: convoluted foam padding. Dimensions: 29 cm x 25 cm x 6 cm (L x W x H). Weight: 510 g
Housing
Robust water-proof cylinder consisting of a polyvinyl chloride (PVC) tube and poly-oxymethylene (POM) endplates. One endplate with 2 male M12 5-pole sockets connected in parallel (MONI-IB4/LAN communication, charging voltage), one male M12 5-pole socket for auxiliaries, and 7 female M12 5-pole sockets (MICRO-PAM measuring head communication)
Dimensions
Cylinder with diameter of 16 cm and length of 24 cm
Data management
Dual data storage on internal 8 MByte circular flash buffer and an industrial grade 512 MByte removable microSD flash card. Wireless data transfer via cellular phone or satellite modem. Online data transfer using RS-485 serial data communication.
Power consumption
5 mW in standby mode. Operating mode, depends on the number of MICRO-PAM measuring heads connected (see MICRO-HEAD/3B power consumption)
Battery
12 V / 7.5 Ah (96 Wh) LiFePO4 battery.
Operating temperature
-30 to +60 °C
Weight
5.4 kg
Design of sensor

Mini quantum sensor for selective PAR (photosynthetically active radiation) measurement, cosine corrected for PPFD (photosynthetical photon flux density) measurement.

Sensor housing

Black anodized aluminum housing

Diffuser material

Perspex

Signal detection

High stability silicone photovoltaic detector with filter set for PAR correction (to learn more about the typical sensitivity see “General Features”). Signal output typically -2 μA / (1000 μmol m-2 s-1)

Temperature coefficient of photodiode

0.01 %/K

Absolute calibration

± 5 %

Angular dependence

error < 4 % between angles from -80° to +80° from normal axis

Immersion coefficient

Typically 1.32

Operating temperature

- 5 °C … + 45 °C

Cable length

3 m

Connector

BNC

Power supply

Not required

Size

Height: 16 mm
Diameter: 14 mm
Diffuser diameter: 5.5 mm

Weight

32 g

Design

Padded plastic case with handle

Dimensions

25 cm x 29 cm x 5 cm (L x W x H)

Weight

680 g

Accessories

Micro quantum sensor
Selective PAR measurement, 0 to 2500 μmol m-2 s-1 PAR
Thermocouple
: Ni-CrNi, -20 to +60 °C
Leaf temperature
-20 to +60 °C
Power supply
JUNIOR-PAM special socket
Cable length
100 cm
Dimensions
13 cm x 2.5 cm (max.) x 5.5 cm (max.) (L x W x H)
Weight
162 g (including cable)