MINI-PAM-II

Version:

Product
New

Photosynthesis Yield Analyzer

MINI-PAM-II

Input

100 V to 240 V AC, 50 to 60 Hz

Output
12 V DC, 5.5 A
Operating temperature
-5 to +45 °C, (non-condensing)
Weight
350 g including cables
Program

WinControl-3 System Control and Data Acquisition Program (Microsoft Windows 10 and 11) for operation of measuring system via PC, data acquisition and analysis. Not compatible with Windows 10 on ARM

Saturation Pulse Analysis

Measured: Ft, F0, FM, F, F0’ (also calculated), FM’. Depending on the leaf clip connected, the software can record PAR, temperature and also humidity. [In the case of the MINI-PAM-II clip humidity can be measured, which the clip of the JUNIOR-PAM cannot.]
Calculated: F0’ (also measured), FV/FM and Y(II) (maximum and effective photochemical yield of PS II, respectively), qL, qP, qN, NPQ, Y(NPQ), Y(NO) and ETR (electron transport rate)

Fitting Routines

Two routines for determination of the cardinal points α, Ik and ETRmax of light curves

Programmed Features

Automatic determination of signal offset for all light intensities and gain levels. Automatic calibration of internal PAR sensor against an external PAR sensor connected to the instrument

Computer Requirements

Processor: 0.8 GHz, RAM: 512 MB, screen resolution: 1024 x 600 pixels, interface: USB 2.0/3.0

Communication Protocol

USB

Design
Metal clip with fiber holder and 11 mm diameter sample hole: 5.5 cm x 1.4 cm (L x W)
Fiber holder
1.2 cm length, mounted 0.7 cm above base, with lateral screw to fix fiber optics. Angle between fiber optics axis and sample plane: 60°. Two spacer rings to vary the distance between fiber end and leaf surface
Measuring light
Blue (470 nm) LED, standard modulation frequencies 5 to 25 Hz adjustable in increments of 5 Hz and 100 Hz, measuring light PAR at standard settings = 0.05 μmol m-2 s-1. Fluorescence at wavelengths greater than 630 nm is measured
Actinic light
Same blue LED as for measuring light, maximum actinic PAR = 3000 μmol m-2 s-1, maximum PAR of saturation pulses = 6000 μmol m-2 s-1 adjustable at increments of 500 μmol m-2 s-1
Measuring light
Red (655 nm) LED, modulation frequencies and PAR as described for MINI-PAM-II/B. Fluorescence at wavelengths greater than 700 nm is measured
Actinic light
Same red LED as for measuring light, maximum PAR of actinic light and saturation pulses as described for MINI-PAM-II/B
Far-red light
Peak emission at 735 nm
Signal detection
PIN photodiode protected by long-pass and a short-pass filters
Data memory
Flash memory, 8 MB, providing memory for more than 27000 saturation pulse analyses
Display
Backlit 160 x 104 dots (78 x 61 mm) transflective B/W LCD display with resistive touchscreen
Ports
Ports for fiberoptics, USB cable, external light source, 2035-B leaf clip, auxiliaries and 12 V DC power supply
Power supply
6 AA (Mignon) rechargeable batteries (Eneloop 1.2 V/2 Ah), providing power for up to 1000 yield measurements; 6 spare batteries, automatic power/off, battery charger (100 to 240 V AC, 50-60 Hz, 0.35 A) for 1 to 8 AA/AAA NI-MH/NI-CD batteries, 12 V 5.5 A power supply MINI PAM-II/N
Operating temperature
-5 to +45 °C (non-condensing)
Dimensions
17.2 cm x 11.2 cm x 7.6 cm (L x W x H)
Weight
1.5 kg (incl. batteries)
Design
Randomized 70 μm glass fibers forming a single plastic-shielded bundle with stainless steel adapter ends
Dimensions
Active diameter 5.5 mm, outer diameter 8 mm, length 100 cm
Weight
180 g
Input
100 to 240 V AC, 50 to 60 Hz
Output
12 V DC, 1.0 A
Operating temperature
-5 to +45 °C (non-condensing)
Dimensions
17.5 cm x 10.5 cm x 3 cm (L x W x H)
Weight
300 g including cable
Design
Aluminum case with custom foam packing
Dimensions
50 cm x 34 cm x 20 cm (L x W x H)
Weight
3.8 kg
Design oxygen meter
Aluminum case with 1 optical sensor port, 1 Pt 100 port, and 1 USB port. Integrated sensors: relative humidity and atmospheric pressure
Dimensions oxygen meter
7.8 cm x 12.0 cm x 2.4 cm
Weight oxygen meter
290 g
Optode
Measuring range: 0% - 500% air saturation (a. s.). Accuracy: ± 0.1% a.s. at 5% a.s. Resolution: 0.05% a.s. at 5% a.s. Response Time: < 3 s for 90% signal change. Minimum Lifetime: 2·106 data points
Design interface box
Aluminum case with USB connector and fixed cable with MINI-PAM-II AUX connector
Dimensions interface box

5.5 cm x 3.0 cm x 2.0 cm

Weight interface box

55 g

Design interface support
Perspex plate with holder for interface connector
Dimensions (max.) interface support
12.0 cm x 11.0 cm x 3.0 cm
Weight interface support

75 g

Design
leaf chamber with a circular 1 cm diameter sample area. Ventilated on one side by air with adjustable flow rate. The amount of water vapor released to the air flow is determined with high precision humidity sensors. The leaf temperature is measured by an IR sensor located in the chamber bottom. GPS information is tracked by a built-in GPS receiver. A mini quantum sensor is positioned on the sample plane. Ambient CO2 values are monitored by a CO2 sensor facing the outside, at the lower left side of the porometer. For chlorophyll a fluorescence measurements, a fiberoptics port aligns the MINI-PAM/F Fiberoptics at an angle of 60° relative to the measuring plane. Including tripod-mount.
Power supply
MINI-PAM-II leaf clip socket; The MINI-PAM-II: the 6 AA (Mignon) rechargeable batteries (Eneloop 1.2 V/2 Ah) provide power for more than 6 hours for typical experiments. The porometer alone can be operated for more than 9 hours at maximum flow. Easy battery swap possible.
Sample area
1 cm diameter
Flow rates
40; 60; 80; 100; 120; 140; 160; 180 or 200 µmol s-1
RH sensor accuracy
typ. 20-70 %RH ±1.0 %RH; <20 %RH and >70 %RH ±1.5 %RH; ΔT = ±0.1 °C
Pressure sensor accuracy
± 0.1 kPa, noise 0.2 Pa
Leaf temp. sensor accuracy
± 0.3 °C, emissivity adjustable 0.1-1.0
Ambient CO2 sensor accuracy
± (30 ppm, + 3 % of reading)
Flowmeter accuracy
± (1.5 % RD + 0.15 % FS)
GPS receiver accuracy
2.0 m CEP (circular error probable)
Micro quantum sensor
Sensor for selective PAR measurement with the spectral properties of the LS-C sensor, range 0 to 7000 µmol m-2 s-1, cosine corrected for light incident at an angle between -30° to +30° from surface normal, internal preamplifier
Parameter
gs mmol m-2 s-1; gt mmol m-2 s-1; gb mmol m-2 s-1; H2Oin mmol mol-1;
dH2O mmol mol-1; H2Oout mmol mol-1; chamber pressure kPa, Temp (leaf) °C; Flow in/out µmol s-1; VPD Pa/kPa; E mmol m-2 s-1; PAR µmol m-2 s-1; GPS-location; GPS-orientation; sun-inclination
Operating conditions
-5 to +45 °C; 0-90 %RH (non-condensing); 30-110 kPa
Cable length
75 cm
Dimensions
24 cm x 7.5 cm x 14 cm (max L x W x H)
Weight
450 g (excluding cable)
Height
Adjustable between 24 cm and 87 cm
Weight
400 g

Accessories

Design
Tube-shaped fiber tip holder composed of polyoxymethylene (POM; 4.5 cm x 2.5 cm, L x D max,) with recessed permanent neodymium magnet. Spring steel band (0.3 mm thick, 1.5 cm wide) consisting of two arms (2 cm and 5 cm, respectively) which form a right angle. The leaf is positioned between the 2 cm arm and the fiber optics tip. The 5 cm arm is attached to the fiber tip holder by the magnet. The 5 cm arm runs in a slit guide. Including a stand with fiberoptics guide
Cuvette

Round stainless steel cuvette (7.5 mm wide, 9.0 mm deep) with top window adapter for connecting the fiberoptics; embedded in PVC body with injection port for Hamilton syringes and hose nozzles for connecting an external flow-through water bath (not included). Including three 6.0 x 1.5 mm magnetic stir bars

Magnetic stirrer
To drive the magnetic flea in the Suspension Cuvette KS-2500; with PVC ring for centering the cuvette and miniature stand to fix the fiberoptics on top of the cuvette
Design
POM tube, at one side, port for light detection, port for fluorescence excitation by blue (452 nm max) or green (525 nm max) LEDs, and port for white light from a tungsten lamp for reflection measurements; at the opposite side, 4-pole underwater socket. Including a 160 cm length, 100 g weight, connection cable with 4-pole underwater plug and 4-pole female plug connecting to the ports for LEAF CLIP or AUX of the MINI-PAM-II
Spectrometer module
Hamamatsu micro-spectrometer, spectral range from 400 to 800 nm, spectral resolution between 8 and 10 nm. Maximum PAR: 4000 µmol m-2 s-1 for illumination having spectral characteristics similar to sunlight
Flat Entrance Optics SPEC/P
Hard-anodized aluminum rod of 10 mm diameter and 50 mm length with internal light guide, at one end with lateral light entrance through a 5 mm diameter diffusor and the opposite end inserted in a mounting plate (diameter 33 mm, height 5 mm)
Fluorescence and Reflection Optics SPEC/R
Spectrometer cap consisting of POM: maximum diameter 35 mm, height 13 mm, weight 16 g. With central 5 mm x 16 mm groove accommodating Perspex light guides for blue and green light for fluorescence excitation, and for white light for reflection measurements. With 3 mm diameter central drilled hole as light channel to the detector window of the spectrometer. Including sample cap to fix sample (maximum diameter 40 mm, height 10 mm, weight 8 g) and 10 mm thick reflectance standard of Zenith Polymer. Spectrometer and sample caps padded with foam rubber, both parts have magnets build in to attract each other and, thus, to hold the sample
Dimensions
3.25 cm diameter, 17.5 cm length max
Weight
135 g
Design
Consisting of a port to insert the MINI-PAM/F Fiberoptics and a clip to hold the sample. A circular 1 cm diameter hole in the upper clip part defines the measuring area. Standard distance between fiberoptics tip and measuring area is 8 mm. The port aligns the fiberoptics at an angle of 60° relative to the measuring plane. A mini quantum sensor is positioned on the sample level by a movable Perspex arm, a leaf temperature sensor is mounted below the sample within an up and down movable Plexiglas tube, and a humidity sensor is mounted at 3 cm distance from the sample. The electronics of the 2035-B unit store calibration factors of sensors. Saturation pulses can be triggered by remote trigger button. An input socket for an additional light sensor is provided
Mini quantum sensor
Sensor for selective PAR measurement with the spectral properties of the LS-C sensor, range 0 to 7000 μmol m-2 s-1, cosine-corrected for light incident at angles between -30° to +30° from surface normal
Thermocouple
Ni-CrNi, wire diameter 0.1 mm, -20 to +60 °C
Humidity sensor
Humidity and temperature sensing integrated circuit, 0 - 100% relative humidity
Power supply
MINI-PAM-II leaf clip socket (5 V/10 mA)
Cable length
110 cm
Dimensions
17 cm x 5.7 cm (max.) x 8 cm (max.) (L x W x H)
Weight
250 g (excluding cable)
Design
Actinic light source attachable on 2035-B Leaf Clip Holder using fixing rods. Light is emitted by a circular 3.3 cm diameter LED array containing 4 RGBW LEDs. The distance between LED array and sample level is 3.5 cm when the 2054-L is mounted on the 2035-B leaf clip
Emission characteristics
Peak at 452 nm, 520 nm, and 630 nm of blue, green, and red light emission, respectively. The white light consists of emissions from 450 nm to 680 nm. The maximum PAR is 1500 µmol m-2 s-1 for each channel (R, G, B, or W). The composition of colors is freely adjustable. The light source is connected to the SYNC port of the MINI-PAM-II fluorometer
Dimensions
(Lamp and fixing rods): 9 cm (max.) x 5 cm (max.) x 11 cm (max.) (L x W x H)
Weight
200 g
Dimensions (without plastic supports)
15 cm x 3.3 cm x 2.5 cm (L x W x H)
Weight
125 g
Design

Aluminum plate (6.0 x 3.3 cm max.) with 11 mm diameter circular hole (measuring area) and aluminum port to position fiber at an angle of 60° relative to the aluminum plate. With port for temperature sensor of 2060-M unit to measure surface temperature and thread to mount the PAR sensor of the 2060-M unit. Connected to a 10 x 0.8 cm (L x Ø) steal rod with two lateral plastic supports (12 cm x 1 cm x 1 cm, L x W x H), which are lockable by knurled screws

Design
Clip made of aluminum with felt contact areas and sliding shutter
Dimensions
6.5 cm x 2 cm (max.) x 1.5 cm (max.) (L x W x H)
Weight
3.6 g
Design
Aluminum clip with 3.2 mm diameter viewing area designed to position small leaves below the fiberoptics of the MINI-PAM-II, prepared to accommodate PAR and temperature sensors of the Mini Quantum/Temp.-Sensor 2065-M
Dimensions
7.6 cm x 3.0 cm (max.) x 5.2 cm (max.) (L x W x H)
Weight
55 g
Mini quantum sensor
LS-C sensor for selective PAR measurement, range 0 to 7000 μmol m-2 s-1, cosine corrected for light incident at angles between -30° to +30° from surface normal
Thermocouple
Ni-CrNi, wire diameter 0.1 mm, -20 to +60 °C
Dimensions of electronic unit
15 cm x 3.3 cm x 2.5 cm (L x W x H)
Weight
125 g (excluding cable)
Design

Single-line handheld laser scanner with trigger button and 1 m, partially coiled cord; to be connected to the Photosynthesis Yield Analyzer MINI-PAM-II or WATER-PAM-II. Bar codes are stored together with fluorescence data

Dimensions
9 cm (max.) x 6 cm (max.) x 16 cm (max.) (L x W x H)
Weight
335 g
Design
Stable tripod for mounting the Standard Measuring Head 3010-S, the tripod fits into the GFS-3000 transport box