MULTI-COLOR-PAM-II

Version:

Product
New

ST-Kinetics and Multi-Wavelength Chlorophyll Fluorometer

MULTI-COLOR-PAM-II

Operating System
Microsoft Windows 10 (32 and 64 bits) and 11; computers that can run these operating systems, can also work with PamWin-4.
Chip-on-board multi-wavelength measuring light LED emitter
400, 440, 480, 540, 590, and 625 nm for pulse-modulated measuring light; 20 intensity settings and 14 settings of pulse frequency
Chip-on-board multi-wavelength actinic LED array
440, 480, 540, 590, 625 and 420-640 nm (white) for continuous actinic illumination, max. 4000 μmol m-2 s-1 PAR; saturating single turnover flashes, max. 200000 μmol m-2 s-1 PAR, adjustable between 5 and 50 μs; multiple turnover flashes, max. 12000 μmol m-2 s -1 PAR, adjustable between 1 and 800 ms
Far-Red LED
peak wavelength 730 nm
Dimensions
10.5 cm x 5.5 cm x 7 cm (L x W x H)
Weight
500 g (incl. cables, 1 m long)
Software
PamWin-4 System Control and Data Acquisition Program for operation of the instrument via PC or laptop, data acquisition and analysis
Fluorescence analysis

Fitting routine for the fast fluorescence O-I1 to determine the functional absorption cross-section of the PS II antennae (Sigma) needed for the determination of PS II-specific electron transport rates

Fitting routine for exponential decay (e.g. QA– reoxidation after a single turnover flash) or rise of a signal with up to three exponentials

Choice of two fitting routines for light curves (determination of cardinal points α, Ik and ETRmax)

Measured

Ft, F0, FM, F, F0' (also calculated), FM'. Fast polyphasic rise and decay kinetics (time resolution up to 10 µs). PAR using Spherical Micro Quantum Sensor US-SQS/WB or Mini Quantum Sensor US-MQS/WB

Calculated

F0' (also measured), Fv/Fm and Y(II) (maximum and effective photochemical yield of PS II, respectively), qL, qP, qN, NPQ, Y(NPQ), Y(NO) and ETR (electron transport rate), C/F0 (constant fraction of F0 that is assumed to originate from PS I)

Measured
Ft, F0, FM, F, F0' (also calculated), FM'. Fast polyphasic rise and decay kinetics (time resolution up to 10 μs). PAR using Spherical Micro Quantum Sensor US-SQS/WB or Mini Quantum Sensor US-MQS/WB.
Calculated
F0' (also measured), FV/FM and Y(II) (maximum and effective photochemical yield of PS II, respectively), qL, qP, qN, NPQ, Y(NPQ), Y(NO) and ETR (electron transport rate), C/F0 (constant fraction of F0 not constituting PS II chlorophyll fluorescence)
Measured
Ft, F0, FM, F, F0’ (also calculated), FM’. Time-resolved Saturation Pulse: Fast polyphasic rise and decay kinetics (time resolution up to 10 µs). PAR using Spherical Micro Quantum Sensor US-SQS/WB or Mini Quantum Sensor US-MQS/WB
Calculated
F0’ (also measured), FV/FM and Y(II) (maximum and effective photochemical yield of PS II, respectively), qL, qP, qN, NPQ, Y(NPQ), Y(NO) and ETR (electron transport rate), C/F0 (constant fraction of F0 not constituting PS II chlorophyll fluorescence)
General design

ARM microcontroller (480 MHz), Fast Kinetics up to 128,000 points with 14 bit resolution, ST Kinetics up to 15,000 points with 14 bit resolution, unlimited storage for Slow Kinetics

Sockets

4 sockets for measuring light and actinic light of MCP-II-E Multi-Color Emitter Head and measuring light/auxiliary detector and ST supply MCP-II-EDST, 2 sockets for signal detection by MCP-II-D1 and MCP-II-D2DST Detector Heads, charge socket or Battery Charger MINI-PAM/L, output socket for PHYTO-MS Miniature Magnetic Stirrer, 4 BNC sockets for 5 V trigger in and trigger out signals and ST trigger in and trigger out signals, input socket for US-SQS/WB Spherical Micro Quantum Sensor or US-MQS/WB Mini Quantum Sensor, input socket for auxiliary devices, USB socket

Communication
USB 2.0, USB 3 compatible
User interface
Windows computer with PamWin-4 software
Power supply

standard: NO sealed lead-acid battery; Battery Charger MINI-PAM/L (100 to 240 V AC)

Dimensions
31 cm x 16 cm x 33.5 cm (W x H x D)
Power consumption
6 W
Weight
3.64 kg
Operating temperature
-5 to +40 °C
Chip-on-board multi-wavelength measuring light LED emitter

400, 440, 480, 540, 590, 625 nm for pulse-modulated measuring light; 20 intensity settings and 14 pulse frequency settings

Chip-on-board multi-wavelength actinic LED array
440, 480, 540, 590, 625 and 420-640 nm (white) for continuous actinic illumination, max. 5000 µmol photons m-2 s-1; saturating single turnover flashes, max. 200000 µmol photons m-2 s-1; multiple turnover pulses, max. 12000 µmol m-2 s-1 PAR, adjustable between 1 and 800 ms
Far-Red LED
peak wavelength 730 nm
Dimensions
10.5 cm x 5.5 cm x 7 cm (L x W x H)
Weight
500 g (incl. cables, 1 m long)
Signal detection
PIN photodiode with special pulse preamplifier for measuring fluorescence changes with maximum time resolution of 10 µs
Filter box
for up to 14 mm filter thickness
Standard detector filter
long-pass filter >650 nm (3 mm RG 665) plus short-pass filter SP 710
Dimensions
6.9 cm x 9.8 cm x 6.4 cm (L x W x H)
Weight
355 g (incl. cables, 1 m long)
Signal generation and detection
High quality 450 nm ST-flashes of variable duration and intensity up to 1 mol photons m-2 s-1; Actinic light (450 nm); Far Red light (740 nm); STK-detector for surface detection of direct fluorescence with sub-µs time resolution
Dimensions
15.4 cm x 5.2 cm x 7 cm
Weight
570 g (incl. cables, 1.2 m long)
STK & PAM-Detector (switchable)

includes: PAM-detector equivalent to MCP-II-D1 and STK-detector for non-modulated fluorescence with sub-µs time resolution

Filter box
for up to 14 mm filter thickness
Standard detector filter
long-pass filter >650 nm (3 mm RG 665) plus short-pass filter SP 710
Dimensions
6.9 cm x 9.8 cm x 6.4 cm (L x W x H)
Weight
550 g (incl. cables, 1.2 m long)
Input
90 to 264 V AC, 47 to 63 Hz
Output
19 V DC, 3.7 A
Operating temperature
0 to 40 °C
Dimensions
15 cm x 6 cm x 3 cm (L x W x H)
Weight
300 g
Design

Aluminum box with custom foam packing

Dimensions

60 cm x 40 cm x 34 cm (L x W x H);
60 liter

Weight

5 kg

Accessories

Design

Black-anodized aluminum body with central 10 x 10 mm glass cuvette; for attachment of Measuring Heads and Miniature Magnetic Stirrer PHYTO-MS; additional ports for attachment of two additional measuring heads

Weight

750 g

Temperature Control Block ED-101US/T
Sectioned block with central 10 x 10 mm opening to be mounted on top of the ED-101US/MD unit; to be connected to external flow-through water bath (not included), weight: 250 g
Miniature Magnetic Stirrer PHYTO-MS

Based on a device manufactures by h+p (type Variomag-Mini); featuring adapter to be mounted in the bottom port of the Optical Unit ED-101US/MD; powered and controlled by the Power-and-Control-Unit

Spherical Micro Quantum Sensor US-SQS/WB

3.7 mm Ø diffusing sphere coupled to integrated PAR sensor via 2 mm diameter fiber; compact amplifier unit and special holder for mounting on Optical Unit ED-101US/MD; to be connected tot the Power-and-Control Unit

Temperature Control Block ED-101US/T
Sectioned block with central 10 x 10 mm opening to be mounted on top of the ED-101US/MD unit; to be connected to external flow-through water bath (not included), weight 250 g
Miniature Magnetic Stirrer PHYTO-MS
Based on device manufactured by h+p (type Variomag-Mini); featuring adapter to be mounted in bottom port of the Optical Unit ED-101US/MD; powered and controlled by the Power-and-Control-Unit DKN-C
Spherical Micro Quantum Sensor US-SQS/WB
3.7 mm ø diffusing sphere coupled to integrated PAR sensor via 2 mm diameter fiber; compact amplifier unit and special holder for mounting on Optical Unit ED-101US/MD; to be connected to the Power-and-Control Unit DKN-C
Design

Magnetic stirrer driven by a rotating magnetic field; the PHYTO-MS is connected to Power-and-Control Unit PHYTO-II-C; a special adapter plug allows the insertion in the bottom port of the Optical Unit ED-101US/MP

Weight
16 g
Display
Three line LCD display
Control range
0 °C to 50 °C at 0.1 K steps
Operating voltage
11 V – 14 V DC
Maximum Peltier current
1 A
Size
105 mm x 90 mm x 130 mm (W x H x D)
Weight
0.57 kg

Power-and-Control Unit US-T/DR

Display
Three line LCD display
Control range
0 °C to 50 °C at 0.1 K steps
Operating voltage
11 V - 14 V DC
Maximum Peltier current
1 A
Size
105 mm x 90 mm x 130 mm (W x H x D)
Weight
0.57 kg

Peltier Heat-Transfer Head US-T/DS

Achievable temperatures
12 K below ambient temperature, 15 K above ambient temperature (Quartz cuvette placed in Optical Unit for Suspensions ED-101US/MD with 1.5 mL water and stirrer PHYTO-MS on)
Size
⌀ 55 mm, 110 mm height
Cable length
130 cm
Weight

0.29 kg (including cable)

AC Adapter

Input

100 V - 240 V AC 1.5 A 50-60 Hz

Output
12 V DC 5.5 A
Size

130 mm x 56 mm x 30 mm (L x W x H)

Weight
0.50 kg (including cable)
Dimensions

Base plate, 40 cm x 30 cm

Height

73.5 cm, diameter 1.5 cm

Weight

2.8 kg