PAM-2500

Version:

Product

High-performance Field and Laboratory Chlorophyll Fluorometer

PAM-2500

Design
Metal clip with fiber holder and 11 mm diameter sample hole: 5.5 cm x 1.4 cm (L x W)
Fiber holder
1.2 cm length, mounted 0.7 cm above base, with lateral screw to fix fiber optics. Angle between fiber optics axis and sample plane: 60°. Two spacer rings to vary the distance between fiber end and leaf surface
Design
Flexible, steel-spiral, plastic-covered bundle with three-pin optical connector
Joint end (measuring site)
Active diameter 6 mm, outer diameter 8 mm
Length
100 cm
Weight
300 g
Operating System
Microsoft Windows 10 (32 and 64 bits) and 11; computers that can run these operating systems, can also work with PamWin-4.
Input
100 to 240 V AC, 47 to 63 Hz
Output
19 V DC, 3.7 A
Operating temperature
0 to 40 °C
Dimensions
15 cm x 6 cm x 3 cm (L x W x H)
Weight
300 g
Height
Adjustable between 24 cm and 87 cm
Weight
400 g
Software
PamWin-4 System Control and Data Acquisition Program for operation of the instrument via PC or laptop, data acquisition and analysis
Fluorescence analysis

Fitting routine for the fast fluorescence O-I1 to determine the functional absorption cross-section of the PS II antennae (Sigma) needed for the determination of PS II-specific electron transport rates

Fitting routine for exponential decay (e.g. QA– reoxidation after a single turnover flash) or rise of a signal with up to three exponentials

Choice of two fitting routines for light curves (determination of cardinal points α, Ik and ETRmax)

Measured

Ft, F0, FM, F, F0' (also calculated), FM'. Fast polyphasic rise and decay kinetics (time resolution up to 10 µs). PAR using Spherical Micro Quantum Sensor US-SQS/WB or Mini Quantum Sensor US-MQS/WB

Calculated

F0' (also measured), Fv/Fm and Y(II) (maximum and effective photochemical yield of PS II, respectively), qL, qP, qN, NPQ, Y(NPQ), Y(NO) and ETR (electron transport rate), C/F0 (constant fraction of F0 that is assumed to originate from PS I)

Mini quantum sensor
LS-C sensor, selective PAR measurement, 0 to 20000 μmol m-2 s-1 PAR
Thermocouple
Ni-CrNi, 0.1 mm diameter,
-20 to +60 °C
Output
PAR, high sensitivity range: 0 to 1000 µmol/(m2·s); normal sensitivity range: 0 to 20000 μmol m-2 s-1 PAR (output 0 to 2.5 V for each range). Leaf temperature, -20 to +60 °C (0 to 0.8 V)
Power supply
PAM-2500 leaf clip socket (5 V/4 mA)
Length of power cable
100 cm
Length of sensor cables
30 cm
Dimensions
16 cm x 3 cm x 1.7 cm (L x W x H)
Weight
220 g
Description
Charger for external battery 000160101314, input 100-230 V, 50-60 Hz AC, 0.4 kg
Signal detection
PIN-photodiode protected by long-pass filter (T50%=715 nm). Selective window amplifier
Sockets
Connector for Special Fiberoptics 2010-F. USB socket. Sockets for Leaf Clip Holder 2030-B or Micro Quantum/Temperature-Sensor 2060-M, Battery Charger MINI-PAM/L or external 12 V battery via MINI-PAM/AK cable, and external lamp/trigger output
Communication
USB. Bluetooth Version 2.0 + EDR Class 2
User interface
Windows computer with PamWin-3 software
Power consumption
Basic operation 1.6 W, 8 W with all internal light sources operated at maximum output (measuring light, red and blue actinic light, and far red light). Saturation pulse at maximum intensity, 30 W
Power supply
Rechargeable sealed lead-acid battery 12 V/2 Ah; Battery Charger MINI-PAM/L (100 to 240 V AC)
Recharging time
6 hours (with the PAM-2500 turned off) via Battery Charger MINI-PAM/L
Operating temperature
-5 to +40 °C
Operating humidity range
20 to 95% RH to avoid condensation
Dimensions
23 cm x 10.5 cm x 10.5 cm (L x W x H) aluminum housing
Weight
2.5 kg (including battery)
Measuring light
Red LEDs, maximum emission at 630 nm, FWHM (full width at half maximum) 20 nm. 1 μs pulses at modulation frequencies 10 to 5000 Hz for F0 determinations (200 Hz default), and 1 to 100 kHz during actinic illumination, fast kinetics with 100 or 200 kHz, 20 intensity levels, frequency dependent effective PAR ranging from 0.001 to 100 μmol m-2 s-1
Blue actinic light
LEDs, maximum emission at 455 nm, FWHM 20 nm, PAR up to 800 μmol m-2 s-1, 20 intensity levels
Red actinic light
LEDs, maximum emission at 630 nm, FWHM 15 nm, PAR up to 4000 μmol m-2 s-1, 20 intensity levels
Saturation pulses
Red LEDs (see red actinic light), PAR up to 25000 μmol m-2 s-1, adjustable between 0.1 and 0.8 s, 20 intensity levels
Multiple turn-over flashes
Red LEDs (see red actinic light), PAR up to 25000 μmol m-2 s-1, adjustable between 1 and 800 ms, 20 intensity levels
Single turn-over flashes
Red LEDs (see red actinic light), PAR up to 125000 μmol m-2 s-1, adjustable between 5 and 50 μs
Far-red light
LED, maximum emission at 750 nm, FWHM 25 nm, 20 intensity levels
Carrier
Robust field carrier bag with shoulder and hip belt
Design

Aluminum box with custom foam packing

Dimensions
60 cm x 40 cm x 25 cm (L x W x H)
Weight
5 kg

Accessories

Design
Tube-shaped fiber tip holder composed of polyoxymethylene (POM; 4.5 cm x 2.5 cm, L x D max,) with recessed permanent neodymium magnet. Spring steel band (0.3 mm thick, 1.5 cm wide) consisting of two arms (2 cm and 5 cm, respectively) which form a right angle. The leaf is positioned between the 2 cm arm and the fiber optics tip. The 5 cm arm is attached to the fiber tip holder by the magnet. The 5 cm arm runs in a slit guide. Including a stand with fiberoptics guide
Cuvette

Round stainless steel cuvette (7.5 mm wide, 9.0 mm deep) with top window adapter for connecting the fiberoptics; embedded in PVC body with injection port for Hamilton syringes and hose nozzles for connecting an external flow-through water bath (not included). Including three 6.0 x 1.5 mm magnetic stir bars

Magnetic stirrer
To drive the magnetic flea in the Suspension Cuvette KS-2500; with PVC ring for centering the cuvette and miniature stand to fix the fiberoptics on top of the cuvette
Dimensions (without plastic supports)
15 cm x 3.3 cm x 2.5 cm (L x W x H)
Weight
125 g
Design

Aluminum plate (6.0 x 3.3 cm max.) with 11 mm diameter circular hole (measuring area) and aluminum port to position fiber at an angle of 60° relative to the aluminum plate. With port for temperature sensor of 2060-M unit to measure surface temperature and thread to mount the PAR sensor of the 2060-M unit. Connected to a 10 x 0.8 cm (L x Ø) steal rod with two lateral plastic supports (12 cm x 1 cm x 1 cm, L x W x H), which are lockable by knurled screws

Design
Clip made of aluminum with felt contact areas and sliding shutter
Dimensions
6.5 cm x 2 cm (max.) x 1.5 cm (max.) (L x W x H)
Weight
3.6 g
Mini quantum sensor
Magnetically attached, swivel-mounted sensor (see LS-C sensor), selective PAR measurement, 0 to 20000 μmol m-2 s-1 PAR
Thermocouple
Ni-CrNi, diameter 0.1 mm, -20 to +60 °C
Output
PAR, high sensitivity range: 0 to 1000 μmol m-2 s-1; normal sensitivity range: 0 to 20000 μmol m-2 s-1 (output 0 to 2.5 V for each range). Leaf temperature, -20 to +60 °C (0 to 0.8 V). Remote trigger button, signal line connected to ground
Power supply
PAM-2500 leaf clip socket (5 V/4 mA)
Cable length
100 cm
Dimensions
17 cm x 5.7 cm (max.) x 8 cm (max.) (L x W x H)
Weight
310 g
Design
Aluminum clip with 3.2 mm diameter viewing area designed to position small leaves below the fiberoptics of the MINI-PAM-II, prepared to accommodate PAR and temperature sensors of the Mini Quantum/Temp.-Sensor 2065-M
Dimensions
7.6 cm x 3.0 cm (max.) x 5.2 cm (max.) (L x W x H)
Weight
55 g
Design

90 cm cable with fuse for over-current protection. The cable bypasses the internal battery of the PAM-2500 fluorometer and supplies voltage from an external 12 V battery directly to the electronics. External DC cable to connect 12 V battery. One red and one black (GND) blade receptacle at one end, the other end with special PAM-2500 EXT. DC plug.

Length

90 cm

 

Weight

45 g

Design
Stable tripod for mounting the Standard Measuring Head 3010-S, the tripod fits into the GFS-3000 transport box