WATER-PAM-II

Version:

Product

Chlorophyll Fluorometer for Phytoplankton

WATER-PAM-II

Input

100 V to 240 V AC, 50 to 60 Hz

Output
12 V DC, 5.5 A
Operating temperature
-5 to +45 °C, (non-condensing)
Weight
350 g including cables
Program

WinControl-3 System Control and Data Acquisition Program (Microsoft Windows 10 and 11) for operation of measuring system via PC, data acquisition and analysis. Not compatible with Windows 10 on ARM

Saturation Pulse Analysis

Measured: Ft, F0, FM, F, F0’ (also calculated), FM’. Depending on the leaf clip connected, the software can record PAR, temperature and also humidity. [In the case of the MINI-PAM-II clip humidity can be measured, which the clip of the JUNIOR-PAM cannot.]
Calculated: F0’ (also measured), FV/FM and Y(II) (maximum and effective photochemical yield of PS II, respectively), qL, qP, qN, NPQ, Y(NPQ), Y(NO) and ETR (electron transport rate)

Fitting Routines

Two routines for determination of the cardinal points α, Ik and ETRmax of light curves

Programmed Features

Automatic determination of signal offset for all light intensities and gain levels. Automatic calibration of internal PAR sensor against an external PAR sensor connected to the instrument

Computer Requirements

Processor: 0.8 GHz, RAM: 512 MB, screen resolution: 1024 x 600 pixels, interface: USB 2.0/3.0

Communication Protocol

USB

Features
Data memory
128 kB CMOS RAM providing memory for 4000 data sets
Microcontroller
CMOS 80C52
Display
2 x 24 character alphanumerical LC-display with backlight
User interface
2 x 4 touch-sensitive keys to operate the internal mode
Measured parameters
F0, FM, FM', F, FV/FM (max. Yield), ΔF/FM' (Yield), qP, qN, NPQ, PAR (using special micro quantum sensor), ETR (i.e. PAR x ΔF/FM')
Power supply
Internal rechargeable battery 12 V/2 Ah, providing power for at least 10 000 Yield measurements, automatic power off, battery charger MINI-PAM/L (100 to 240 V AC)
Dimensions
17.6 cm x 11.5 cm x 9.5 cm (L x W x H)
Weight
2 kg (incl. battery)
Operating temperature
-5 to +45 °C
Connectors
Actinic light
Drives up to three actinic LEDs
AUX input
Receives signals from temperature and light sensors
AUX output
Sends control signals to external LED drivers
Charge
Socket for battery charger
Far-red light
Up to three far-red LEDs can be driven
Measuring light
Capacity to drive up to three LEDs, both as measuring and actinic light sources, 12 LED intensity and 12 pulse frequency settings are available, automatic change of measuring light frequency
Output
Provides analog signal to chart recorder
Photomultiplier
Full control of photomultiplier PM-MF
RS232
Communication with Windows Computer
Computer-controlled Operation
Software
WinControl-3 System Control and Data Acquisition Program (Microsoft Windows 10 and 11) for operation of measuring system via PC, data acquisition and analysis. Not compatible with Windows 10 on ARM
Input
100 to 240 V AC, 50 to 60 Hz
Output
12 V DC, 1.0 A
Operating temperature
-5 to +45 °C (non-condensing)
Dimensions
17.5 cm x 10.5 cm x 3 cm (L x W x H)
Weight
300 g including cable
Height
Adjustable between 24 cm and 87 cm
Weight
400 g
Design
Metal housing with LED array, photomultiplier, pulse signal preamplifier and cables connecting to photomultiplier (PM) and AUX output sockets of PAM‑CONTROL unit.
LED Array
Two red measuring light LEDs (620 nm) and two red actinic light LEDs (635 nm), all equipped with a filter blocking radiation > 700 nm. 1 blue LED (460 nm) for selective excitation of photosystem I in cyanobacteria. Note: the blue LED of the WATER-EDF1.5R is controlled by the Far-Red LED switch in the WinControl-3 software. Actinic intensity up to 2000 µmol quanta m‑2 s‑1 of photosynthetically active radiation (PAR). Saturation pulse intensity up to 4000 µmol quanta m‑2 s‑1.
Dimensions: 11.5 cm x 9 cm x 5.5 cm (L x W x H)
Weight: 550 g (incl. cable 0.6 m long)
Signal detection
Photomultiplier detector based on Photosensor Module H‑6779‑01 (Hamamatsu) with high red sensitivity; featuring pulse preamplifier and automatic overload switch-off; fluorescence detection at wavelengths > 710 nm
Fiber Optics
Three 1.5 mm diameter plastic fiber optics (lengths 200 mm, 600 mm, and 1200 mm) for connection with the emitter-detector box are supplied.
Dimensions
11 cm x 9 cm x 8 cm (L x W x H)
Weight
600 g (incl. cable 0.6 m long)
LED Array
Two blue measuring light LEDs (460 nm) and two blue actinic light LEDs (460 nm). One far red LED (730 nm) for selective excitation of photosystem I in non-cyanobacterial photosynthetic organisms. Actinic intensity up to 2000 µmol quanta m‑2 s‑1 of photosynthetically active radiation (PAR). Saturation pulse intensity up to 4000 µmol quanta m‑2 s‑1.
Signal detection
As Emitter-Detector-Fiberoptics Unit (red) WATER-EDF1.5R but fluorescence detection at wavelengths > 680 nm.
Other specifications
See Emitter-Detector-Fiberoptics Unit (red) WATER-EDF1.5R.
Design
Bottom cylinder, holding the emitter-detector unit, black polyoxymethylene plastic (POM) with lateral plastic screw for fixation of emitter-detector unit, 600 g, 10 cm x 11 cm (height x diameter). Top cylinder, encircling measuring chamber, POM, 160 g, 6 cm x 10 cm (height x diameter). Cover, POM, 0.8 cm x 10 cm (thickness x diameter). Cuvette holder, to be mounted on emission window of emitter-detector unit, POM, 5 g, 3.8 cm x 1.9 cm x 0.6 cm (L x W x H), 1.5 cm diameter central hole for placing round fluorescence cuvette WATER-K.
General Features
Data memory
128 kB CMOS RAM providing memory for 4000 data sets
Microcontroller
CMOS 80C52
Display
2 x 24 character alphanumerical LC-display with backlight
User interface
2 x 4 touch-sensitive keys to operate the internal mode menu
Measured parameters
F0, FM, FM', F, FV/FM (max. Yield), ΔF/FM' (Yield), qP, qN, NPQ, PAR (using special micro quantum sensor), ETR (i.e. PAR x ΔF/FM')
General design
Power supply
Internal rechargeable battery 12 V/2 Ah, providing power for at least 10 000 Yield measurements, automatic power/off, battery charger MINI-PAM/L (100 to 240 V AC)
Dimensions
17.6 cm x 11.5 cm x 9.5 cm (L x W x H)
Weight
2 kg (incl. battery)
Operating temperature
-5 to +45 °C
Connectors
Actinic light
Drives up to three actinic LEDs
AUX input
Receives signals from temperature and light sensors
AUX output
Sends control signals for external LED drivers
Charge
Socket for battery charger
Far-red light
Up to three far red LEDs can be driven
Measuring light
Capacity to drive up to three LEDs both as measuring and actinic light
sources, 12 LED intensity and 12 pulse frequency settings are available, automatic change
of measuring light frequency.
Output
Provides analog signal to chart recorder
Photomultiplier
Full control of photomultiplier PM-MF
RS232
Data transfer to computer using WinControl V. 2 software
Computer-controlled Operation
Software
WinControl-2 (Microsoft Windows 3.1x/9x/Me/NT4/2000/XP). WinControl-3 (Microsoft Windows XP/Vista/7/8/10)
Measuring light
Blue (450 nm) and red (630 nm) LED, standard modulation frequencies 5 to 25 Hz, adjustable in increments of 5 Hz, and 100 Hz, measuring light PAR at standard settings = 0.05 µmol m-2 s-1. For differential algae analysis auxiliary LEDs 520 and 660 nm.
Actinic light
Blue (450 nm) and red (630) LED as for measuring light, maximum actinic PAR = 3000 µmol m-2 s-1, maximum PAR of saturation pulses = 6000 µmol m-2 s-1 adjustable in increments of 500 µmol m-2 s-1 expandable on request to 12000 µmol m-2 s-1 adjustable in increments of 1000 µmol m-2 s-1
Far-red light
Peak emission at 730 nm
Signal detection
Hamamatsu photomultiplier protected by long-pass and a short-pass filters
Cuvette holder
for 15 mm Ø cuvette, Perspex inlet for overspill protection, POM cuvette cover 35 mm high x 40 mm Ø
Data memory
Flash memory, 8 MB, providing memory for more than 27,000 saturation pulse analyses
Display
Backlit 160x104 dots (78 x 61 mm) transflective B/W LCD display with resistive touchscreen
Ports
PAR sensor, Temp, AUX1, AUX2, SYNC, Stirrer, CTRL1, CTRL2, COMP, EXT DC and USB
Power supply
8 AA (Mignon) rechargeable batteries (Eneloop 1.2 V/2 Ah), providing power for up to 1000 yield measurements; 8 spare batteries, automatic power/off, battery charger (100 to 240 V AC, 50-60 Hz, 0.35 A) for 1 to 8 AA NI-MH batteries, 12 V 5,5 A power supply MINI PAM-II/N
Operating temperature
-5 to +45 °C (non-condensing)
Dimensions
17.2 cm x 13.5 cm x 7.6 cm (L x W x H)
Weight
1.5 kg (incl. battery)
Design
Aluminum case with custom foam packing
Dimensions
50 cm x 34 cm x 20 cm (L x W x H)
Weight
3.8 kg
Application

Suspension cuvette

Connects to

WATER-PAM, DUAL-PAM-100, 
ULM-500 (AUX), PAM-2500 and MINI-PAM (instead of Leaf Clip Holder 2030-B or 2060 M)

Connector

Same as Leaf Clip Holder 2030-B Includes hood for suspension cuvette, amplifier for aux input of PAM Control

Signal output

0…2.5 V DC / 0…1000 μmol m-2 s-1 or 0…2.5 V DC / 0…20.000 μmol m-2 s-1 Power
Provided by connected instrument

Cable length
3 m + 0.5 m
Size

Sensor: Diameter 1 cm
Length: 11 cm
Hood: Diameter 3.4 cm height: 3.2 cm
Amplifier: 5 x 5 x 5 cm (W x L x H)

Weight

175 g

Design of sensor

Mini quantum sensor for selective PAR (photosynthetically active radiation) measurement, cosine corrected for PPFD (photosynthetical photon flux density) measurement.

Sensor housing

Black anodized aluminum housing

Diffuser material

Perspex

Signal detection

High stability silicone photovoltaic detector with filter set for PAR correction (to learn more about the typical sensitivity see “General Features”). Signal output typically -2 μA / (1000 μmol m-2 s-1)

Temperature coefficient of photodiode

0.01 %/K

Absolute calibration

± 5 %

Angular dependence

error < 4 % between angles from -80° to +80° from normal axis

Immersion coefficient

Typically 1.32

Operating temperature

- 5 °C … + 45 °C

Cable length

3 m

Connector

BNC

Power supply

Not required

Size

Height: 16 mm
Diameter: 14 mm
Diffuser diameter: 5.5 mm

Weight

32 g

Accessories

Design

Single-line handheld laser scanner with trigger button and 1 m, partially coiled cord; to be connected to the Photosynthesis Yield Analyzer MINI-PAM-II or WATER-PAM-II. Bar codes are stored together with fluorescence data

Dimensions
9 cm (max.) x 6 cm (max.) x 16 cm (max.) (L x W x H)
Weight
335 g
Design
Cosine-response mini quantum sensor for selective measurement of photosynthetically active radiation (PAR, 400 – 700 nm) with Perspex diffuser disk as light entrance and signal amplifier box
Signal detection
High stability silicone photovoltaic detector with filter set providing equal response to photon fluxes across the PAR spectral range. The typical signal output of the detector is 2 μA / (1000 μmol m‑2 s‑1)
Temperature coefficient of photodiode
0.01 %/K
Absolute calibration
± 5 %
Angular dependence
error < 4 % between angles from -80° to +80° from normal axis
Immersion coefficient
Typically 1.32
Cable length
3 m
Size: Height
16 mm; diameter: 14 mm; Diffuser diameter: 5.5 mm
Weight
32 g
Amplifier box
Two amplification ranges (0 to 1000 and 0 to 20,000 μmol m‑2 s‑1, each range corresponding to 0 to 2.5 V DC). To be connected to the Leaf Clip Holder 2030-B port of the PAM-CONTROL unit. Power provided by the PAM-CONTOL unit. Dimensions: 5 cm x 3 cm x 5 (W x H x D). Weight: 200 g.
Design
Set of 10 perspex stirring paddle for WATER-II/S
Design
Cuvette holder made from black polyoxymethylene polymer (POM) with in-/out water tubing connectors (4 mm inner diameter; 6 mm outer diameter). The cuvette is mounted to the optical unit of the instrument using an adapter ring and two knurled screws.
Dimensions
: Ø 49 mm, with connectors and cuvette 64 mm x 74 mm
Weight
150 g
Design
Tripod holder with 3/8 inch thread (without tripod). WATER-PAM-II mounting is secured with knurled screws. Side resting option for the stirrer and 6 cuvettes.
Dimensions
24.5 x 13 x 4.5 cm
Weight
312 g
Design
Instrument operated stirrer with disposable perspex stirring paddles. Delivery includes a set (10 pieces) of Stirring Paddles WATER-R.
Dimensions
71 mm x 30 mm (l x Ø)
Weight
95 g