WATER-PAM-II

Version:

Product

Chlorophyll Fluorometer for Phytoplankton

WATER-PAM-II (Fiber Version - WATER-PAM)

Input

100 V to 240 V AC, 50 to 60 Hz

Output
12 V DC, 5.5 A
Operating temperature
-5 to +45 °C, (non-condensing)
Weight
350 g including cables
Program

WinControl-3 System Control and Data Acquisition Program (Microsoft Windows 10 and 11) for operation of measuring system via PC, data acquisition and analysis. Not compatible with Windows 10 on ARM

Saturation Pulse Analysis

Measured: Ft, F0, FM, F, F0’ (also calculated), FM’. Depending on the leaf clip connected, the software can record PAR, temperature and also humidity. [In the case of the MINI-PAM-II clip humidity can be measured, which the clip of the JUNIOR-PAM cannot.]
Calculated: F0’ (also measured), FV/FM and Y(II) (maximum and effective photochemical yield of PS II, respectively), qL, qP, qN, NPQ, Y(NPQ), Y(NO) and ETR (electron transport rate)

Fitting Routines

Two routines for determination of the cardinal points α, Ik and ETRmax of light curves

Programmed Features

Automatic determination of signal offset for all light intensities and gain levels. Automatic calibration of internal PAR sensor against an external PAR sensor connected to the instrument

Computer Requirements

Processor: 0.8 GHz, RAM: 512 MB, screen resolution: 1024 x 600 pixels, interface: USB 2.0/3.0

Communication Protocol

USB

Features
Data memory
128 kB CMOS RAM providing memory for 4000 data sets
Microcontroller
CMOS 80C52
Display
2 x 24 character alphanumerical LC-display with backlight
User interface
2 x 4 touch-sensitive keys to operate the internal mode
Measured parameters
F0, FM, FM', F, FV/FM (max. Yield), ΔF/FM' (Yield), qP, qN, NPQ, PAR (using special micro quantum sensor), ETR (i.e. PAR x ΔF/FM')
Power supply
Internal rechargeable battery 12 V/2 Ah, providing power for at least 10 000 Yield measurements, automatic power off, battery charger MINI-PAM/L (100 to 240 V AC)
Dimensions
17.6 cm x 11.5 cm x 9.5 cm (L x W x H)
Weight
2 kg (incl. battery)
Operating temperature
-5 to +45 °C
Connectors
Actinic light
Drives up to three actinic LEDs
AUX input
Receives signals from temperature and light sensors
AUX output
Sends control signals to external LED drivers
Charge
Socket for battery charger
Far-red light
Up to three far-red LEDs can be driven
Measuring light
Capacity to drive up to three LEDs, both as measuring and actinic light sources, 12 LED intensity and 12 pulse frequency settings are available, automatic change of measuring light frequency
Output
Provides analog signal to chart recorder
Photomultiplier
Full control of photomultiplier PM-MF
RS232
Communication with Windows Computer
Computer-controlled Operation
Software
WinControl-3 System Control and Data Acquisition Program (Microsoft Windows 10 and 11) for operation of measuring system via PC, data acquisition and analysis. Not compatible with Windows 10 on ARM
Input
100 to 240 V AC, 50 to 60 Hz
Output
12 V DC, 1.0 A
Operating temperature
-5 to +45 °C (non-condensing)
Dimensions
17.5 cm x 10.5 cm x 3 cm (L x W x H)
Weight
300 g including cable
Height
Adjustable between 24 cm and 87 cm
Weight
400 g
Design
Metal housing with LED array, photomultiplier, pulse signal preamplifier and cables connecting to photomultiplier (PM) and AUX output sockets of PAM‑CONTROL unit.
LED Array
Two red measuring light LEDs (620 nm) and two red actinic light LEDs (635 nm), all equipped with a filter blocking radiation > 700 nm. 1 blue LED (460 nm) for selective excitation of photosystem I in cyanobacteria. Note: the blue LED of the WATER-EDF1.5R is controlled by the Far-Red LED switch in the WinControl-3 software. Actinic intensity up to 2000 µmol quanta m‑2 s‑1 of photosynthetically active radiation (PAR). Saturation pulse intensity up to 4000 µmol quanta m‑2 s‑1.
Dimensions: 11.5 cm x 9 cm x 5.5 cm (L x W x H)
Weight: 550 g (incl. cable 0.6 m long)
Signal detection
Photomultiplier detector based on Photosensor Module H‑6779‑01 (Hamamatsu) with high red sensitivity; featuring pulse preamplifier and automatic overload switch-off; fluorescence detection at wavelengths > 710 nm
Fiber Optics
Three 1.5 mm diameter plastic fiber optics (lengths 200 mm, 600 mm, and 1200 mm) for connection with the emitter-detector box are supplied.
Dimensions
11 cm x 9 cm x 8 cm (L x W x H)
Weight
600 g (incl. cable 0.6 m long)
LED Array
Two blue measuring light LEDs (460 nm) and two blue actinic light LEDs (460 nm). One far red LED (730 nm) for selective excitation of photosystem I in non-cyanobacterial photosynthetic organisms. Actinic intensity up to 2000 µmol quanta m‑2 s‑1 of photosynthetically active radiation (PAR). Saturation pulse intensity up to 4000 µmol quanta m‑2 s‑1.
Signal detection
As Emitter-Detector-Fiberoptics Unit (red) WATER-EDF1.5R but fluorescence detection at wavelengths > 680 nm.
Other specifications
See Emitter-Detector-Fiberoptics Unit (red) WATER-EDF1.5R.
Design
Aluminum case with custom foam packing
Dimensions
50 cm x 34 cm x 20 cm (L x W x H)
Weight
3.8 kg

Accessories

Design

Single-line handheld laser scanner with trigger button and 1 m, partially coiled cord; to be connected to the Photosynthesis Yield Analyzer MINI-PAM-II or WATER-PAM-II. Bar codes are stored together with fluorescence data

Dimensions
9 cm (max.) x 6 cm (max.) x 16 cm (max.) (L x W x H)
Weight
335 g
Design
Cosine-response mini quantum sensor for selective measurement of photosynthetically active radiation (PAR, 400 – 700 nm) with Perspex diffuser disk as light entrance and signal amplifier box
Signal detection
High stability silicone photovoltaic detector with filter set providing equal response to photon fluxes across the PAR spectral range. The typical signal output of the detector is 2 μA / (1000 μmol m‑2 s‑1)
Temperature coefficient of photodiode
0.01 %/K
Absolute calibration
± 5 %
Angular dependence
error < 4 % between angles from -80° to +80° from normal axis
Immersion coefficient
Typically 1.32
Cable length
3 m
Size: Height
16 mm; diameter: 14 mm; Diffuser diameter: 5.5 mm
Weight
32 g
Amplifier box
Two amplification ranges (0 to 1000 and 0 to 20,000 μmol m‑2 s‑1, each range corresponding to 0 to 2.5 V DC). To be connected to the Leaf Clip Holder 2030-B port of the PAM-CONTROL unit. Power provided by the PAM-CONTOL unit. Dimensions: 5 cm x 3 cm x 5 (W x H x D). Weight: 200 g.