MF-F
Accessory
Working Fiber
In the case of fluorescence measurements, one of the fibers guides excitation light to the sample and fluorescence back to the photomultiplier. This fiber can be connected to a “working fiber” (MF-F) which is a single fiber with one free end of 100 μm diameter. The free end can be pulled out to form a tapered tip of 10 to 30 μm. By means of a micromanipulator, this tip can be advanced into different layers of plant tissues to measure photosynthesis gradients. Similarly, light attenuation within a sample can be assessed using a pointed working fiber.
