General Features and Graphical User Interface
The KLAS-100 software used for DUAL-KLAS-NIR measurements is written in the tradition of the DUAL-PAM-100 software. It allows the user to make the sample quickly ready for online signal deconvolution and the measurements the user has in mind via a fixed sequence of steps. A script is provided to determine the maximum P700, PC and Fd signal amplitudes (comparable to the Pm determination of the DUAL-PAM-100). Four NIR wavelength pairs are measured and the P700, PC and Fd contributions to these NIR signals are determined using so-called differential model plots or DMPs. Differential Model Plots for Hedera helix are included in the software for reference. In practice, separate sets of DMPs have to be determined for individual plant species. Via a fixed sequence of steps, making use of the scripts included in the software for the determination of each DMP (P700, PC and Fd), the user can make his or her own set of DMPs for the photosynthetic organism that is studied.
The software has a window for saturation pulse analysis and one for light curves, just like in the DUAL-PAM-100 software, but then including an analysis of the PC and Fd redox states. Like for the DUAL-PAM-100, the software automatically calculates classical fluorescence ratio parameters as well as more recently suggested parameters. In a new window it is possible to plot the P700 and PC redox states against each other, which gives an idea about the redox-equilibrium between the two. Trigger files can be created and Script files written that in combination allow the reproducible execution of complex experimental protocols. Whereas some background knowledge is required for programming Trigger files and Scripts, even very sophisticated protocols can be reliably executed by non-experts. The software gives the user the flexibility to create almost any experimental protocol that can be imagined for the analysis of electron transport related processes.