Specifications for MICRO-PAM ONLINE-Configuration

All specifications are subject to change without prior notice.

Measuring Head MICRO-HEAD/3B

General Design

Housing: Polymer housing with optical block and fiber connector on one side, and a combined power line/RS485 socket on the opposite side

MICRO-HEAD/BK Leaf Clip: Consisting of 2 aluminum frames (3.5 x 2.5 cm), held together by magnet, 0.4 cm distance between sample plane of leaf clip and lightguide, angle between sample plane and optical light guide: 60°

Cables: RS 485 data/power cable, 10 m standard length, connecting MICRO-HEAD/3B and MICRO IB4 PC Interface Box. RS 485 adapter cable, 0.6 m long, to connect MICRO-HEAD/3B to optional PC Interface Box MONI IB4/LAN or to optional MONI DA system for data acquisition

Flexible stand: Baseplate of acrylic glass 10 x 10 x 0.5 cm (L x W x H); flexible goose neck consisting of 20 links, 1.7 cm maximum diameter, 30 cm length, one end with metal thread equipped with 1 butterfly nut, 1 nut and 2 washers. Weight 114 g

Dimensions: Complete head with leaf clip, 13.5 x 3.5 x 4 cm (L x W x H)

Power consumption: Peak loads during saturating pulses 3 W. During measuring mode 0.15 W

Operating temperature: -15 to +40 °C

Weight: 96 g

Light Emission

Modulated fluorescence excitation: Blue power LED (typical peak wavelength 470 nm, full width at half maximum 22 nm). Photosynthetically active radiation (PAR) of measuring light at level of the sample clip range from 0.15 to 1.5 μmol m-2 s-1 at low modulation frequencies (5 to 25 Hz), and from 1.5 to 22.5 μmol m-2 s-1 at high modulation frequencies (100 Hz)

Actinic light: Same power LED as for modulated light. At sample level of leaf clip, 3000 μmol m-2 s-1 maximum PAR of actinic light, 8000 μmol m-2 s-1 maximum PAR of saturating flashes


Fluorescence: PIN-photodiode protected by longpass filter (50% transmittance at 645 nm). Selective window amplifier to measure pulse amplitude modulated (PAM) fluorescence.

Photosynthetically active radiation (PAR): External LS-C sensor for selective PAR measurement, range 0 to 7000 μmol m-2 s-1, cosine-corrected for light incident at angles between -30° to +30° from the surface normal

Temperature: Thermocouple: Ni-CrNi, wire diameter 0.1 mm, -20 to +60 °C

Humidity: Capacitive-type humidity sensor on a specialized analog and digital integrated circuit, 0 - 100% relative humidity

PC Interface Box MONI-IB4/LAN

Housing: Aluminum case with RS-232, USB-B, Ethernet, power supply sockets, and four M12 5-pole sockets for RS-485 communication

Interfacing: The interface box connects a computer with up to four MICRO-HEAD/3B (or one MONI-DA). RS-485 serial data communication is used between interface box and MICRO-HEAD/3B or MONI-DA. RS232, USB or Ethernet communication is used between interface box and computer

Recommended maximum cable lengths: To computer via USB and RS-232: 2 m. To computer via Ethernet, 100 m. To MICRO-HEAD/3B via RS-485: 10 m. To MONI-DA via RS-485, 100 m

Dimensions: 12 x 9.3 x 3 cm (L x W x H)

Weight: 400 g

Operating temperature: 0 to +40 °C

Power supply: Input: 100 to 240 V AC, 50 to 60 Hz. Output: 19 V DC, 3.7 A. Dimensions: 13.2 x 5.8 x 3 cm (L x W x H). Weight: 310 g

Fluorometer Software and Computer Minimum Requirements

Program: WinControl-3 System Control and Data Acquisition Program (Microsoft Windows 10 and 11) for operation of measuring system via PC, data acquisition and analysis. Not compatible with Windows 10 on ARM

Saturation Pulse Analysis: WinControl-3 System Control and Data Acquisition Program (Windows 7, 8, 10) for operation of the MICRO-PAM system, data acquisition and data analysis

Program: Measured: Ft, F0, FM, F, F0' (also calculated), FM'. Calculated: F0' (also measured), FV/FM and Y(II) (maximum and effective photochemical yield of PS II, respectively), qL, qP, qN, NPQ, Y(NPQ), Y(NO) and ETR (electron transport rate). Fitting Routines: Two routines for determination of the cardinal points α, Ik and ETRmax of light curves

Further date acquired: PAR, leaf temperature, humidity

Additional feature: Automatic determination of signal offset for all light intensities and all gain levels

Communication Protocol: USB

Computer Requirements: Processor, 1 GHz. RAM, 512 MB. Screen resolution, 1024 x 600 pixels. Interface, USB 2.0/3.0

Flexible Ball Joint Mount for MICRO-PAM Measuring Head MICRO-HEAD/SG

Design: Two double-ball arm segments, two ball end pieces with photo thread (1/4” external), to mount a measuring head and a base plate with photo thread (1/4" internal), respectively.

Dimensions: 39 cm (L max.)

Weight: 410 g

Transport Box

Design: Aluminum box with custom foam packing for MICRO-PAM

Dimensions: 60 cm x 40 cm x 25 cm (L x W x H); 42 liter

Weight: 4.7 kg