Specifications for MULTI-COLOR-PAM

Power-and-Control-Unit MCP-C

General design: Microcontroller: 2 x AVR-RISC (8 MHz) + 4 MB SRAM; 256000 data points with 12 bit resolution can be stored

Sockets: 2 sockets for measuring light and actinic light of MCP-E Multi-Color Emitter Head, socket for signal detection by MCP-D Detector Head, charge socket or Battery Charger MINI-PAM/L, output socket for PHYTO-MS Miniature Magnetic Stirrer, BNC sockets for 5 V trigger-in and trigger out signals, input socket for US-SQS/WB Spherical Micro Quantum Sensor or US-MQS/WB Mini Quantum Sensor, input socket for auxiliary devices, connector for optional PS I lamp, USB socket

Communication: USB 2.0 and USB 3.0 compatible

User interface: Windows computer with PamWin-3 software

Power supply: Rechargeable sealed lead-acid battery 12 V/2 Ah; Battery Charger MINI-PAM/L (100 to 240 V AC)

Dimensions: 31 cm x 16 cm x 33.5 cm (W x H x D), aluminum housing with carrying handle

Power consumption: Basic operation 1.6 W, 8 W with all internal light sources operated at maximum output. Saturation Pulse at maximum intensity, 30 W

Weight: 2.5 kg (including battery)

Operating temperature: -5 to +40 °C

Multi-Color Emitter Head MCP-E

Chip-on-board multi-wavelength measuring light LED emitter: 400, 440, 480, 540, 590, and 625 nm for pulse-modulated measuring light; 20 intensity settings and 14 settings of pulse frequency

Chip-on-board multi-wavelength actinic LED array: 440, 480, 540, 590, 625 and 420-640 nm (white) for continuous actinic illumination, max. 4000 μmol m-2 s-1 PAR; saturating single turnover flashes, max. 200000 μmol m-2 s-1 PAR, adjustable between 5 and 50 μs; multiple turnover flashes, max. 12000 μmol m-2 s -1 PAR, adjustable between 1 and 800 ms

Far-Red LED: peak wavelength 730 nm

Dimensions: 10.5 cm x 5.5 cm x 7 cm (L x W x H)

Weight: 500 g (incl. cables, 1 m long)

Detector Head MCP-D

Signal detection: PIN photodiode with special pulse preamplifier for measuring fluorescence changes with maximum time resolution of 10 μs

Filter box: For up to 14 mm filter thickness

Standard detector filter: long-pass filter > 650 nm (3 mm RG 665) plus short-pass filter SP 710

Dimensions: 6.9 cm x 9.8 cm x 6.4 cm (L x W x H)

Weight: 355 g (incl. cables, 1 m long)

Battery Charger MINI-PAM/L

Input: 90 to 264 V AC, 47 to 63 Hz

Output: 19 V DC, 3.7 A

Operating temperature: 0 to 40 °C

Dimensions: 15 cm x 6 cm x 3 cm (L x W x H)

Weight: 300 g

Optical Unit for Suspensions ED-101US/MD

Design: Black-anodized aluminum body with central 10 x 10 mm standard glass cuvette; for attachment of Measuring Heads MCP-E and MCP-D, and Miniature Magnetic Stirrer PHYTO-MS; two additional ports for attachment of two additional devices

Weight: 750 g

Stand ST-101

Stand for mounting the Optical Units ED-101US/MD (suspensions) or MCP BK (leaves)

Transport Box PHYTO-T

Design: Aluminum box with custom foam packing for MULTI-COLOR-PAM and accessories

Dimensions: 60 cm x 40 cm x 34 cm (L x W x H)

Weight: 5 kg

System Control and Data Acquisition

Software: PamWin-3 System Control and Data Acquisition Program for operation of measuring system via PC, data acquisition and analysis

Saturation Pulse Analysis

Measured: Ft, Fo, Fm, F, Fo' (also calculated), Fm'. Fast polyphasic rise and decay kinetics (time resolution up to 10 μs). PAR using Spherical Micro Quantum Sensor US-SQS/WB or Mini Quantum Sensor US-MQS/WB.

Calculated: Fo' (also measured), Fv/Fm and Y(II) (maximum and effective photochemical yield of PS II, respectively), qL, qP, qN, NPQ, Y(NPQ), Y(NO) and ETR (electron transport rate), C/Fo (constant fraction of Fo not constituting PS II chlorophyll fluorescence)

Fitting Routines

Fitting routine for fast fluorescence rise from 0 to the I1 level to determine functional absorption cross-section of PS II and PS II-specific electron transport rates. Fitting of fluorescence decay after light-to-dark transition by up to three exponentials to estimate primary electron transfer rates. Fitting routine for determination of the cardinal points α, Ik and ETRmax of light curves.

Computer Requirements

Processor, 0.8 GHz. RAM, 512 MB. Screen resolution, 1024 x 600 pixels. Interface, USB 2.0/3.0. Operating system: Microsoft Windows XP/Vista/7/8/10