IMAGING-PAM

Version:

Product

Chlorophyll Fluorescence System

IMAGING-PAM (Microscopy Version - IMAGING-PAM M-Series)

Design

Modified AxioScope 5 Microscope (Zeiss) adapted for IMAGING PAM applications. Comprises binocular fototube (30°/23 100:0/0:100), condenser 0,9/1,25H and transmitted light unit HAL 50. Detector filter RG665, dichroic mirror 420-640 nm, video adapter 60N-C 2/3" 0,5x and standard lens Fluar 20x are already mounted

Design
Reflector Module with filter set for additional microscope LED lamp modules. Consisting of a beam splitter filter (420- 640 nm) and a detector filter (665 nm), mounted in a Zeiss reflector module frame
Design
Aluminum housing featuring built-in Li-ion battery, sockets for cable connections with CCD Cameras IMAG-K6 or IMAG-K7, connectors for the MAXI, MINI and MICROSCOPY Measuring Heads and Battery Charger 2120-N
Microcontroller
RISC processor
User interface

For Windows 10 and 11 PCs with ImagingWinGigE software versions; connection is via GigE ethernet

Power supply
Internal rechargeable Li-ion battery 14.8 V/5.2 Ah
Power consumption
9 W (500 mA) drawn from internal Li-ion battery
Recharging time
Approx. 4 hours (IMAGING-PAM turned off) via Battery Charger 2120-N
Operating temperature
-5 to +45 °C
Dimensions
25 cm x 10.5 cm x 11 cm (L x W x H)
Weight
2.1 kg (incl. internal battery)
Design

Black and white C-mount camera operated in 11-bit-mode at 16 frames/sec featuring 2 x 2 pixel binning

CMOS Chip

2/3" readout (1392 x 1040 px primary resolution; 640 x 480 px effective resolution after binning)

Interface

GigE-Vision®

Lens Mount

C-mount

Dimensions

2.9 cm x 2.9 cm x 4.1 cm (L x W x H) (without lens and cooling bodies)

Weight

65 g (camera only)

Input
90 to 264 V AC, 47 to 63 Hz
Output
19 V DC, 3.7 A
Operating temperature
0 to 40 °C
Dimensions
15 cm x 6 cm x 3 cm (L x W x H)
Weight
300 g
Minimum PC requirements

Intel core i5 or comparable CPU, min 8 GB free RAM, built-in Gigabit Ethernet (GigE), Win11 OS

Features
Data display and evaluation plus instrument settings on 7 different windows:
Design

Box 1: Aluminum box with individual foam lining for IMAGING-PAM MICROSCOPY version accessories

Box 2: Sturdy cardboard box with individual foam lining for Zeiss Axioscope 5 and accessories

Dimensions

Box 1: 60 cm x 40 cm x 35 cm (L x W x H)

Box 2: 68 cm x 62 cm x 64 cm (L x W x H)

Weight

Box 1: 5 kg

Box 2: 5 kg

Accessories

Design
Microscope LED Lamp Module 470 nm (blue) for fluorescence excitation of Chl fluorescence of most algae groups. Emission peak at 470 nm. Shipped together with a set of neutral grey filters for using the system together with higher magnifications.
Design
Microscope LED Lamp Module 625 nm (red-orange) for fluorescence excitation of Chl fluorescence of most algae groups including cyanobacteria. Emission peak at 625 nm. Shipped together with a set of neutral grey filters for using the system together with higher magnifications.
Design

Red-Green-Blue Microscopy LED Lamp allowing computer-assisted deconvolution of major algae groups. Fluorescence excitation and actinic illumination using red (620 nm), green (520 nm), blue (460 nm) or white light (mixed 620, 520 and 460 nm); featuring fluid light guide (100 cm length, 3 mm active dia.), connecting to collimator optics at excitation port of epifluorescence microscope; with cable to be connected to RGB output socket at IMAG-CG; featuring printed circuit board with separate drivers for RGB LEDs;

Design

Light grey plastic housing with connectors, membrane keyboard and a white illuminated LCD graphic display

Dimensions

12 x 7.5 x 3.5 cm

Weight

210 g (including 4 AAA 1.5 V batteries)

Power supply

4 AAA-type batteries or 5 V DC from USB voltage source when connected to the computer

Working conditions

up to 85 % rH (avoid condensation), - 20° to + 50°C ambient temperature

Time resolution

PAR channel #1: 100 samples / second, PAR channel #2 and other channels: 5 samples / second (connected to computer running WinControl-3 software)

Operation time

10 days or ca. 100 days automated logging with sleep mode (1 meas. / 5 min). Unlimited working time via USB connection (PC-software WinControl-3 – no sleep mode)

Inputs
  • Two BNC-connectors for the connection of two PAR-sensors with individual calibration factors between -50.0 and -9999.9 (memory for 10 calibration factors), range switchable in 5 steps 250 nA to 0.6 mA
  • Connector for Monitoring Leaf-Clip JUNIOR-B; an adapter is available for the connection of the Leaf-Clip Holder 2030-B, and the Micro Quantum/Temp.-Sensor 2060-M
  • Connector for additional digital sensors (still under development)
  • USB-Connector for connection with computer (software: WinControl-3)
Memory

Flash memory used as ring buffer for 50000 lines (1 line / single measurement)

Display

White illuminated graphic display with 5 different display modes (1: all data; 2-4: two selected sensors in big letters; 5: chart mode for channel no. 1, with maximum, minimum and average indicated), resolution: 0.1 μmol m-2 s-1

Computer Connection

1 free USB socket. Processor, 1 GHz. RAM, 256 MB. Hard disc space, 20 MB. Screen resolution: 800 x 600 pixels. Interface, USB 1.1, 2.0 and 3.0. Operating system: Microsoft Windows 10 and 11.

Design of sensor

Microscopy quantum sensor for PPFD (photosynthetical photon flux density) measurement.

Sensor housing

Black anodized aluminum housing

Diffuser material

Resin with fluorescence dye

Signal detection

High stability silicon photovoltaic. Signal output typically 1.5 μA / (1000 μmol m-2 s-1)

Temperature coefficient of photodiode

0.01 %/K

Absolute calibration

± 5 %

Operating temperature

0 °C … + 40 °C

Cable length

55 cm

Connector

BNC

Power supply

Not required

Size

Height: 5.5 mm
Length: 76 mm
Width: 26 mm
Diffuser diameter: 0.2 mm

Weight
36 g