Specifications for JUNIOR-PAM

All specifications are subject to change without prior notice.

Optoelectronic Unit JUNIOR-PAM (BLUE Version)

Modulated fluorescence excitation: Blue LED (wavelength of maximum emission: 445 nm). Modulation frequencies 5 to 25 Hz adjustable in increments of 5 Hz, and 100 Hz. Fluorescence at wavelengths greater than 630 nm is measured

Actinic light: Same LED as used for modulated light. Actinic PAR, 25 to 1500 μmol μmol m-2 s-1; maximum PAR of saturation pulses, 10 000 μmol μmol m-2 s-1. All data valid for 1 mm distance from the tip of the 50 cm JUNIOR-PAM light guide

Optoelectronic Unit JUNIOR-PAM/W (WHITE Version)

Modulated fluorescence excitation: White LED (wavelengths of maximum emission: 445 and 545 nm). Modulation frequencies 5 to 25 Hz adjustable in increments of 5 Hz, and 100 Hz. Fluorescence at wavelengths greater than 650 nm is measured

Actinic light: Same LED as used for modulated light. PAR at 1 mm distance from the tip of the 50 cm JUNIOR-PAM light guide: between 25 and 1500 μmol m-2 s-1. Maximum PAR of saturation pulses, 10 000 µmol m-2 s-1

Optoelectronic Unit JUNIOR-PAM and JUNIOR-PAM/W

Housing: Aluminum housing with USB type B socket, M8 4-pole socket to connect the JUNIOR-PAM monitoring leaf clip (accessory), port for JUNIOR-PAM light guide, and swivel-mounted sample support

Far-red light: LED with 745 nm maximum emission wavelength for selective excitation of photosystem I

Fluorescence detection: PIN photodiode protected by long-pass filter. Selective window amplifier to measure pulse-amplitude modulated (PAM) fluorescence

Communication: Standard USB communication via 1.2 m USB-cable type A-B

Power supply: 5 V DC supplied by the USB-cable type A-B used for communication

Power consumption: 100 mW at normal operation and 500 mW during saturation pulse

Dimensions: 11.5 cm x 6.5 cm x 3 cm (L x W x H)

Weight: 200 g

Operating temperature: 0 to + 40 °C

Operating humidity range: 35 to 85% RH (to avoid condensation)

Software WinControl-3

Program: WinControl-3 System Control and Data Acquisition Program (Microsoft Windows 10) for operation of measuring system via PC, data acquisition and analysis

Saturation Pulse Analysis: Measured: Ft, F0, FM, F, F0’ (also calculated), FM’. PAR, leaf temperature and relative humidity using Monitoring Leaf-Clip JUNIOR-BD. Calculated: F0’ (also measured), FV/FM and Y(II) (maximum and effective photochemical yield of PS II, respectively), qL, qP, qN, NPQ, Y(NPQ), Y(NO) and ETR (electron transport rate)

Fitting Routines: Two routines for determination of the cardinal points α, Ik and ETRmax of light curves

Programmability: Fully programmable using batch file language

Computer Requirements: 1 free USB socket. Processor, 1 GHz. RAM, 512 MB. Screen resolution, 1024 x 600 pixels. Interface, USB 2.0/3.0

Miscellaneous items included

Light guide: 50 cm x 1.5 mm (length x diameter) plastic fiber

Sample clips: Open leaf clip (angle between incident radiation from JUNIOR-PAM and leaf surface, 60°) and magnetic leaf clip (angle between incident radiation from JUNIOR-PAM and leaf surface, 90°)

Fluorescence standard: 3 cm x 2 cm fluorescence foil

Polishing set: Set for fiber tips including various polishing pads

USB cable: A-B USB cable

Software CD: Including latest WinControl-3 software

Transport Case JUNIOR-T: Grey plastic box with handle. Interior: convoluted foam padding. Dimensions: 29 cm x 25 cm x 6 cm (L x W x H). Weight: 510 g

Accessories

Monitoring Leaf-Clip JUNIOR-BD

Micro quantum sensor: Selective PAR measurement, 0 to 2500 μmol m-2 s-1 PAR

Thermocouple: Ni-CrNi, -20 to +60 °C

Leaf temperature: -20 to +60 °C

Power supply: JUNIOR-PAM special socket

Cable length: 100 cm

Dimensions: 13 cm x 2.5 cm (max.) x 5.5 cm (max.) (L x W x H)

Weight: 162 g (including cable)

Extra-long Light-Guide

100 cm x 1.5 mm (length x diameter) plastic fiber for difficult accessible samples or underwater measurements