PHYTO-PAM-II

PHYTO-PAM-II Operation Software

General Features and Graphical User Interface
PhytoWin-3 is the dedicated control and data analysis software for PHYTO-PAM-II instruments. Designed to run on Windows PCs, it provides full operational control of the instrument and supports the setup, execution, and analysis of various fluorescence measurement protocols.

The nine software tabs are:

Channels: Raw data, fluorescence data at 5 different excitation wavelengths

Settings: Control tab for settings such as measuring pulse frequency, actinic intensity, saturation pulse width and intensity, clock interval, etc.

Algae: Deconvoluted fluorescence data for cyanobacteria, green algae, diatoms/dinoflagellates and phycoerythrin-containing organisms (e.g. cryptophytes) based on reference excitation spectra; user interface for Chl a determination

Light Curve: Graphic display of light response curves; effective quantum yield and relative electron transport rate (ETR) as a function of PAR, Curve fitting based on a modified Eilers & Peeters (1988) or Platt (1980) model

Slow Kinetics: Graphic display of slow kinetics recorded in the SP-analysis mode

Report: Shows all the measured data and instrument settings stored, export of data for other programs

Reference: Recording and display of reference excitation spectra of cyanobacteria, green algae, diatoms/dinoflagellates and phycoerythrin-containing organisms

Fluo Spec: Display of measuring light characteristics and non-normalized reference excitation spectra

Fast Kinetics: Control and graphical display of fast kinetics analysis providing information on the wavelength-dependent absorption cross-section of PS II, σII(λ)

Free software updates will keep your instrument always up-to date on latest developments.
PhytoWin-3 cannot be used to operate first generation PHYTO-PAM instruments.

Operation Software for the FIBER Version of the PHYTO-PAM

FIBER Version instruments belong to the first generation PHYTO-PAM, which are controlled by PhytoWin software on a Windows PC. Please note that these four-wavelength PHYTO-PAM models are not compatible with PhytoWin-3 software.

PhytoWin provides a user-friendly interface with seven dedicated tabs—Channels, Algae, Report, Light Curve, Settings, Reference, and Delta F—that support both the visualization and analysis of raw and processed fluorescence data. These modules allow for a comprehensive evaluation of photosynthetic parameters and phytoplankton group composition of up to three algal groups.