PHYTO-PAM-II

Version:

Product

Multiple Excitation Wavelength Phytoplankton & Photosynthesis Analyzer

What Does the PHYTO-PAM-II Do?

Natural water contains a mixture of different algae. Standard fluorometers measure them all together as one signal. The PHYTO-PAM-II goes further: it separates the contribution of up to four algal groups within one sample and analyzes their photosynthetic performance individually.

This is possible because different algae groups have different light-harvesting pigments. By exciting the sample with five different wavelengths (440, 480, 540, 590, 625 nm), the PHYTO-PAM-II generates a fluorescence excitation spectrum that is unique to each group. The built-in software uses reference spectra from pure cultures to deconvolute the mixed signal - automatically, in real time.

Ultra-Sensitive Photomultiplier Detection

The PHYTO-PAM-II uses a photomultiplier detector that reaches sensitivity levels down to 0.1 µg Chl a/L, which is typical for oligotrophic lakes and open ocean water. This means the instrument works with natural, unprocessed water samples without the need for pre-concentration.

Comprehensive Photosynthesis Analysis

Beyond composition, the PHYTO-PAM-II provides a full PAM fluorescence analysis for each algae group: saturation pulse quenching analysis, effective PSII quantum yield, light response curves, and induction curves. A fast kinetics mode also allows determination of the wavelength-dependent PSII absorption cross section (σII) for single cultures.

Two Versions

The Compact version integrates all components in a single housing. Lightweight, battery-powered, and ideal for fieldwork and on-deck use.

The Modular version separates emitter, detector, and control unit around an open optical unit. This allows custom filter sets, temperature control, and additional optical ports - designed for advanced laboratory applications.

Scientific Publications using Walz Devices

Source: Google Scholar.
Keywords: (Walz OR Waltz) Effeltrich.
Date: June 22, 2026.

Ʃ = 19642

Per Year

Source: Google Scholar.
Keywords: (Walz OR Waltz) Effeltrich.
Date: June 22, 2026.

Ʃ = 19642

Year

Selected Publications

Study on the treatment of livestock and poultry wastewater using algae-bacteria symbiotic system: effect of inoculation proportion and performance

Ding Q, Zhou Z, Cui L, Liu J, You G, Chen Q, Hou J, Fan X, Yang Y

Environmental Technology 46: 2597-2614

Go to publication

Competition for light color between marine Synechococcus strains with fixed and variable pigmentation

Dufour L, Garczarek L, Mattei F, Gouriou B, Clairet J, Ratin M, Kehoe DM, Huisman J, Verspagen JMH, Partensky F

Applied and Environmental Microbiology 91

Go to publication

The ability of hydrogen peroxide (H2O2) to degrade saxitoxin-, microcystin-, anatoxin-, and non-toxin-producing strains of the harmful cyanobacterium Dolichospermum

Gobler CJ, Kramer BJ, Lusty MW, Thraen J, McTague S

Journal of Environmental Management 387: 125696

Go to publication

Application of a dynamic energy budget model to the blood clam, Tegillarca granosa, reared in culture pond

He L, Jiang B, Zheng X, Yuan J, Lin Z

Aquaculture Reports 40: 102602

Go to publication

Comparative analysis of microalgae's physiological responses to fibrous and layered clay minerals

Jiang Z, Wei T, Wu S, Wang Z, Zhao Z, Zhang L, Ge Y, Li Z

Biology 14: 647

Go to publication

Nitrogen excess induces cytokinesis arrest and alters lipid metabolism in the bloom-forming desmid Cosmarium tinctum

Kang S, Kim K-H, Shin HH, Kim J-H, Kim B-H, Li Z

Water Research X 28: 100394

Go to publication

The harmful cyanobacterium Microcystis aeruginosa differently affects the growth rate and photosynthetic efficiency of several species of marine phytoplankton

Pak NY, Choi HS, Kang SU, Lim AS

Environmental Microbiology Reports 17: e70091

Go to publication

Impact of nutritional history, prey quality, and quantity on grazing and photophysiological responses in the mixoplanktonic dinoflagellate Karenia brevis

Ahn SH, Mayali X, Weber PK, Glibert PM

Limnology and Oceanography 70: 2603-2617

Go to publication

The Great Lakes winter grab: limnological data from a multi-institutional winter sampling campaign on the Laurentian Great Lakes

Pu G, Shchapov K, Pearce NJT, Bowen K, Bramburger A, Camilleri A, Carrick H, Chaffin JD, Cody W, Coleman ML, Currie WJS, Depew DC, Doubek JP, Eveleth R, Fitzpatrick M, Glyshaw PW, Godwin CM, McKay RM, Munawar M, Niblock H, Quintanilla M, Rennie M, Sand MW, Schraitle KJ, Twiss MR, Uzarski DG, Vanderploeg HA, Vick-Majors TJ, Westrick JA, Wheelock BA, Xenopoulos MA, Zastepa A, Ozersky T

Limnology and Oceanography Letters 10: 37-61

Go to publication

Eco-friendly novel algicidal efficiency of Streptomyces erocidicus JXJ-0089 against harmful algal bloom of Microcystis aeruginosa MDEG1

Al-Taywi AJM, Mohammed MH, Al-Taywi NJM, Jassim RJ, Moussa HR

Egyptian Journal of Aquatic Biology & Fisheries 29: 945-961

Impact of thermal fluctuations on phytoplankton: an experimental multi-trait analysis across species

Tascón-Peña O, Cabrerizo MJ, Pérez-Lorenzo M, Marañón E

Journal of Plankton Research 47: fbaf021

Go to publication

Influence of temperature and nutrients on the inhibitory effects of the submerged macrophyte Egeria densa on the growth and saxitoxin prouduction of the cyanobacterium Raphidiopsis raciborkii

Melo Cabral JV, dos Santos Severiano J, dos Santos-Silva RN, Bezerra Nery AM, Santos de Araújo-Silva M, Otogo RA, Chia MA, de Lucena Barbosa JE

Journal of Applied Phycology 37: 1967-1980

Go to publication

Homeostatic response of calcium and potassium ions in Microcystis spp. and Chlorella to MC-LR

Cheng C, Steinman AD, Xue Q, Shu Y, He S, Cai Y, Cai Y, Xie L

Ecotoxicology and Environmental Safety 304: 119144

Go to publication

The effects of temperature and sulfamethoxazole on the growth and photosynthetic characteristics of Phaeodactylum tricornutum.

Xia J, Bao Y, Gao Y, Li J

Marine Pollution Bulletin 200: 116122

Go to publication

Phytoplankton diversity, spatial patterns, and photosynthetic characteristics under environmental gradients and anthropogenic influence in the Pearl River estuary.

Xia J, Hu H, Gao X, Kan J, Gao Y, Li J

Biology 13: 550

Go to publication

Targeted cultivation of diatoms in mariculture wastewater by nutrient regulation and UV-C irradiation.

Shen J, Zheng X, Liu M, Xu K, He L, Lin Z

Frontiers in Microbiology 15: 1371855

Go to publication

The carbonate system of Penzhina Bay and the Shelikhov Gulf in the sea of Okhotsk during extreme tides in summer.

Semkin P, Baigubekov K, Barabanshchikov Y, Gorin S, Koltunov A, Sagalaev S, Ulanova O, Tishchenko P, Shvetsova M, Shkirnikova E, Tishchenko P, Zhang J

Journal of Marine Science and Engineering 12: 517

Go to publication

Ecological investigations of giant Phaeocystis colonies in Vietnam: II. Photosynthesis – irradiance characteristics and nitrogen uptake.

Meng R, Smith WO, Cao R, Doan-Nhu H, Nguyen-Ngoc L

Journal of Phycology 60: 1273-1284

Go to publication

Management of eutrophication using combined the ‘flock [&] sink’ mitigation technique and submerged plants restoration: a mesocosm study.

Liu Y, Liu J, Chen Y, Dai T, Li W, Xu J, Zhang X, Tang L, Zheng F, Zhong J

Environmental Research Communications 6: 055012

Go to publication

Ferric- and calcium-loaded red soil assist colonization of submerged macrophyte for the in-situ remediation of eutrophic shallow lake: from mesocosm experiment to field enclosure application.

Li W, Dai T, Liu J, Zhong J, Wu K, Gao G, Chen Y, Fan H

Science of the Total Environment 924: 171730

Go to publication

Mesocosm study of PAC-modified clay effects on Karenia brevis cells and toxins, chemical dynamics, and benthic invertebrate physiology.

Devillier, VM, Hall ER, Lovko V, Pierce R, Anderson DM, Lewis KA

Harmful Algae 134: 102609

Go to publication

Transcriptomic insights into the shift of trophic strategies in mixotrophic dinoflagellate Lepidodinium in the warming ocean.

Chen J, Deng L, Pang M, Li Y, Xu Z, Zhang X, Liu H

ISME Communications 4: ycae087

Go to publication

Time of day of infection shapes development of a eukaryotic algal-Nucleocytoviricota virocell.

Chase EE, Truchoni AR, Creasey BA, Wilhelm SW

FEMS Microbiology Ecology 100: fiae123

Go to publication

Temporal patterns of stream biofilm in a mountain catchment: one-year monthly samplings across streams of the Orobic Alps (Northern Italy).

Bonacina L, Fornaroli R, Mezzanotte V, Marazzi F

Hydrobiologia 851: 2081-2097

Go to publication

Development of a fluorescence measurement system capable of rapid red tide monitoring.

Baek K-H, Oh Y, Cho H, Kang Y, Lee J-S

Journal of Sensor Science and Technology 33: 30-33

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Temperature-dependent mixotrophy in natural populations of the toxic dinoflagellate Karenia brevis.

Ahn SH, Glibert PM

Water 16: 1555

Go to publication

Discovery of a high-efficient algicidal bacterium against Microcystis aeruginosa based on examinations toward culture strains and natural bloom samples.

Zhang H, Xie Y, Zhang R, Zhang Z, Hu X, Cheng Y, Geng R, Ma Z, Li R

Toxins 15: 220

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Ocean acidification alters the benthic biofilm communities in intertidal soft sediments.

Zhai C, Liang Y, Yu H, Ji Y, Chen X, Wang M, McMinn A

Frontiers in Marine Science 10: 1117826

Go to publication

Effects of dissolved organic matter from sediment and soil samples on the growth and physiology of four bloom-forming algal species.

Xiang R, Zheng B, Jia H

Ecotoxicology and Environmental Safety 263: 115266

Go to publication

Assessing the potential of Chlorella sp. phycoremediation liquid digestates from brewery wastes mixture integrated with bioproduct production.

Wang S, Zhao Q, Yu H, Du X, Zhang T, Sun T, Song W

Frontiers in Bioengineering and Biotechnology

Go to publication

In situ H2O2 treatment of blue green algae contaminated reservoirs causes significant improvement in drinking water treatability.

Melo Rocha MA, Clemente A, Amorim Santos A, da Silva Melo J, Pestana CJ, Lawton LA, Capelo-Neto J

Chemosphere 333: 138895

Go to publication

Summer phytoplankton photosynthetic characteristics in the Changjiang river estuary and the adjacent East China Sea.

Li J, Gao Y, Gao X, Glibert PM

Frontiers in Marine Science 10: 1111557

Go to publication

Statewide assessment reveals spatiotemporal variability of iron in Iowa lakes.

Leung T, Swanner ED

Journal of Contemporary Water Research & Education 177: 2-16

Go to publication

Heatwaves, elevated temperatures, and a pesticide cause interactive effects on multi-trophic levels of a freshwater ecosystem.

Hermann M, Peeters ETHM, van den Brink PJ

Environmental Pollution 327: 121498

Go to publication

Acclimation to various temperature and pCO2 levels does not impact the competitive ability of two strains of Skeletonema marinoi in natural communities.

Briddon CL, Nicoară M, Hegedüs A, Niculea A, Bellerby R, Eikrem W, Crespo BG, Dupont S, Drugă B

Frontiers in Marine Science 10: 1197570

Go to publication

Comparative assessment of algaecide performance on freshwater phytoplankton: understanding differential sensitivities to frame cyanobacteria management.

Allaf MM, Erratt KJ, Peerhossaini H

Water Research 234: 119811

Go to publication

In hot water: interactions of temperature, nitrogen form and availability and photosynthetic and nitrogen uptake responses in natural Karenia brevis populations.

Ahn SH, Glibert PM, Heil CA

Harmful Algae 129: 102519

Go to publication

Morphological, physiological, and transcriptional responses of the freshwater diatom Fragilaria crotonensis to elevated pH conditions.

Zepernick BN, Niknejad DJ, Stark GF, Truchon AR, Martin RM, Rossignol KL, Paerl HW, Wilhelm SW

Frontiers in Microbiology 13: 1044464

Go to publication

Seasonal phytoplankton and geochemical shifts in the subsurface chlorophyll maximum layer of a dimictic ferruginous lake.

Swanner ED, Wüstner M, Leung T, Pust J, Fatka M, Lambrecht N, Chmiel HE, Strauss H

MicrobiologyOpen 11: e1287

Go to publication

Antarctic lake phytoplankton and bacteria from near-surface waters exhibit high sensitivity to climate driven disturbance.

Sherwell S, Kalra I, Li W, McKnight DM, Priscu JC, Morgan-Kiss RM

Environmental Microbiology 12: 6017-6032

Go to publication

Chlorophyll and phycocyanin in-situ fluorescence in mixed cyanobacterial species assemblages: Effects of morphology, cell size and growth phase.

Rousso BZ, Bertone E, Stewart R, Aguiar A, Chuang A, Hamilton DP, Burford MA

Water Research 212: 118127

Go to publication

Photosynthesis and pigment production: elucidation of the interactive effects of nutrients and light on Chlamydomonas reinhardtii.

Mogany T, Bhola V, Ramanna L, Bux F

Bioprocess and Biosystems Enigineering 4: 187-201

Go to publication

Microalgal treatment of the effluent from the hydrothermal carbonization of microalgal biomass.

Mantovani M, Collina E, Marazzi F, Lasagni M, Mezzanotte V

Journal of Water Process Engineering 49: 102976

Go to publication

Synergistic promoting effect of increasing aquatic ammonium and CO2 on Microcystis aeruginosa.

Ma J, Wang P, Hu B, Wang X, Qian J

Chemosphere 301: 134553

Go to publication

Repeated hydrogen peroxide dosing briefly reduces cyanobacterial blooms and microcystin while increasing fecal bacteria indicators in a eutrophic pond.

Lusty MW, Gobler CJ

Journal of Environmental Sciences 124: 522-543

Go to publication

Efeito da luz de LED branca, vermelha, azule e verde em parâmetros da fisiologia da cianobactéria Aphanocapsa holsatica: taxa de crescimento, producão de biomoléculas e biofixação do CO2.

Lima AEV

Thesis Federal University of Sao Carlos

Algicidal mechanism of Raoultella ornithinolytica against Microcystis aeruginosa: antioxidant response, photosynthetic system damage and microcystin degradation.

Li D, Kang X, Chu L, Wang Y, Song X, Zhao X, Cao X

Environmental Pollution 287:117644

Go to publication

Physiological responses of chlorophyta microalgae under environmentally relevant copper concentrations: biomolecules, oxidative stress and photosynthesis.

Dauda S

Thesis Federal University of Sao Carlos

Hydrolysis of organophosphorus by diatom purple acid phosphatase and sequential regulation of cell metabolism.

Wang X, Balamurugan S, Liu S-F, Ji C-Y, Liu Y-H, Yang W-D, Jiang L, Li H-Y

Journal of Experimental Botany 72: 2918-2932

Go to publication

Effect of hydrogen peroxide on natural phytoplankton and bacterioplankton in a drinking water reservoir: mesocosm-scale study.

Santos AA, Guedes DO, Barros MUG, Oliveira S, Pacheco ABF, Azevedo SMFO, Magalhães VF, Pestana CJ, Edwards C, Lawton LA, Capelo-Neto J

Water Research 197: 117069

Go to publication

A comparison of decimeter scale variations of physical and photobiological parameters in a late winter first-year sea ice in Southwest Greenland.

Lund-Hansen LC, Petersen CM, Haubjerg Søgaard D, Sorell BK

Journal of Marine Science and Engineering 9: 60

Go to publication

In-situ rapid monitoring of phytoplankton community to investigate role of iron during cyanobacterial dominance.

Leung T

PhD-thesis Iowa State University 288651060

Iron availability allows sustained cyanobacterial blooms: a dual-lake case study.

Leung T, Wilkinson GM, Swanner ED

Inland Waters 11: 417-429

Go to publication

Absorption and speciation of arsenic by microalgae under arsenic-copper co-exposure.

Huang Z, Chen B, Zhang J, Yang C, Wang J, Song F, Li S

Ecotoxicology and Environmental Safety 213: 112024

Go to publication

GPCR genes as activators of surface colonization pathways in a model marine diatom.

Fu W, Chaiboonchoe A, Dohai B, Sultana M, Baffour K, Alzahmi A, Weston J, Al Khairy D, Daakour S, Jaiswal A, Nelson DR, Mystikou A, Brynjolfsson S, Salehi-Ashtiani K

iScience 23: 101424

Go to publication

Assessment of anammox, microalgae and white-rot fungi-based processes for the treatment of textile wastewater.

Bellucci M, Marazzi F, Musatti A, Fornaroli R, Turolla A, Visigalli S, Bargna M, Bergna G, Canziani R, Mezzanotte V, Rollini M, Ficara E

PLoS ONE 16: e0247452

Go to publication

Interactions between microalgae and bacteria in the treatment of wastewater from milk whey processing.

Marazzi F, Bellucci M, Fantasia T, Ficara E, Mezzanotte V

Water 12: 297

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Photosynthetic efficiency and nutrient physiology of the diatom Thalassiosira pseudonana at three growth temperatures.

Gleich SJ, Plough LV, Glibert PM

Marine Biology 167: 124

Go to publication

Assessing disinfection byproduct risks for algal impacted surface waters and the effects of peracetic acid pre-oxidation.

Kralles ZT, Ikuma K, Dai N

Environmental Science: Water Research & Technology 6: 2365-2381

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Rapid and positive effect of bicarbonate addition on growth and photosynthetic efficiency of the green microalgae Chlorella sorokiniana (Chlorophyta, Trebouxiophyceae).

Salbitani G, Bolinesi F, Affuso M, Carraturo F, Mangoni O, Carfagna S

Applied Sciences 10: 4515

Go to publication

Optimization of combined submerged macrophyte planting conditions for inhibiting algae by response surface methodology.

Wang S, Yu J, Guo F, Pan G, Zhang L, Hu H, Lu Y, Dao G

Water 12: 2093

Go to publication

Different ecological mechanisms lead to similar grazer controls on the functioning of periphyton Antarctic and sub-Antarctic communities.

Valdivia N, Pardo LM, Macaya EC, Huovinen P, Gómez I

Progress in Oceanography 174: 7-16

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Cyanobacteria in eutrophic waters benefit from rising atmospheric CO2 concentrations.

Ma J, Wang P, Wang X, Paerl HW

Science of the Total Environment 691: 1144-1154

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Mixotrophy with multiple prey species measured with a multiwavelength-excitation PAM fluorometer: case study of Karlodinium veneficium.

Lin C-H, Glibert PM

Journal of Plankton Research 41: 46-62

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Harmful algae in Chesapeake Bay: A study focused on Karlodinium veneficum applying time series, physiological, and modeling approaches.

Lin M

PhD-thesis, University of Maryland, USA

Go to publication

Remote sensing of albedo-reducing snow algae and impurities in the Maritime Antarctica.

Huovinen P, Ramírez J, Gómez I

ISPRS Journal of Photogrammetry and Remote Sensing 146: 507-517

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Effects of sediment deposition on periphytic biomass, photosynthetic activity and algal community structure.

Izagirre O, Serra A, Guasch H, Elosegi

Science of The Total Environment 407: 5694-5700

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Community-level microalgal toxicity assessment by multiwavelength-excitation PAM fluorometry.

Schmitt-Jansen M, Altenburger R

Aquatic Toxicology 86: 49-58

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Chlorophyll content and fluorescence responses cannot be used to gauge reliably phytoplankton biomass, nutrient status or growth rate.

Kruskopf M and Flynn KJ

New Phytologist 169: 525-536

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Estimation of chlorophyll content and daily primary production of the major algal groups by means of multiwavelength-excitation PAM chlorophyll fluorometry: performance and methodological limits.

Jakob T, Schreiber U, Kirschesch V, Langner U, Wilhelm C

Photosynthesis Research 83: 343-361

Go to publication

Phosphate regime structures species composition in cultured phototrophic biofilms.

van der Grinten E, Janssen M, Simis SGH, Barranguet C, Admiraal W

Freshwater Biology 49: 369-381

Go to publication

F0-spectra of chlorophyll fluorescence for the determination of zooplankton grazing.

Lürling M, Verschoor AM

Hydrobiologia 491: 145-157

Go to publication

Allelopathic growth inhibition of selected phytoplankton species by submerged macrophytes.

Körner S and Nicklisch A

Journal of Phycology 38: 862-871

Go to publication

Estimation of primary production with Phyto-PAM-fluorometry.

Nicklisch A and Köhler J

Annual Report of the Institute of Freshwater Ecology and Inland Fisheries Berlin 13: 47-60

Chlorophyll fluorescence: New instruments for special applications

Schreiber U

Photosynthesis: Mechanisms and Effects (Garab G. ed) Vol. V, pp. 4253-4258

Chlorophyll fluorescence analysis of cyanobacterial photosynthesis and acclimation.

Campbell D, Hurry V, Clarke AK, Gustafsson P and Öquist G

Microbiology and Molecular Biology Reviews 62: 667-683

Go to publication

Computer-controlled phytoplankton analyzer based on 4-wavelengths PAM chlorophyll fluorometer.

Kolbowski J and Schreiber U

Photosynthesis: From Light to Biosphere.(Mathis P. ed.), Vol. V, pp. 825-828

PHYTO-PAM-II

General design
Metal housing for PHYTO-PAM-II Power-and-Control-Unit including all opto-electronic components as well as the measuring chamber for 15 mm Ø quartz cuvette WATER-K.
Chip-on-board multi-wavelength measuring light LED emitter
440, 480, 540, 590, and 625 nm for pulse-modulated measuring light; 2 intensity settings; 8 settings of pulse frequency and 3 settings of auto MF-high pulse frequency
Chip-on-board multi-wavelength actinic LED array
440, 480, 540, 590, 625 and 420-640 nm (white) for continuous actinic illumination, up to 1500 µmol m-2 s-1 PAR; fast kinetic flashes up to 7000 µmol m-2 s-1 PAR; saturation pulse up to 5000 µmol m-2 s-1 PAR,
Far-Red LED
peak wavelength 725 nm
Signal detection

Photomultiplier detector

Standard detector filter
long-pass filter > 650 nm
Sockets
charge socket for Battery Charger MINI-PAM/L, input socket for US-SQS/WB Spherical Micro Quantum Sensor, USB socket
Communication
USB 1.1, USB 2.0 and USB 3.0 compatible
User interface
Windows computer with PhytoWin-3 software
Power supply
Rechargeable sealed lead-acid battery 12 V/2 Ah; Battery Charger MINI-PAM/L (100 to 240 V AC)
Dimensions
29 cm x 30 cm x 20.5 cm (l x w x h), aluminum housing with carrying handle and cuvette cover
Power consumption

Basic operation 1.5 W, ML +AL at maximum output 4.5 W. Saturation Pulse at maximum intensity, 7 W

Weight
4.8 kg (including battery)
Operating temperature
-5 to +40 °C
Design
Cuvette holder from black polyoxymethylene plastic (POM) with in-/out water tubing connectors (4 mm inner Ø and M 5 screw), to be mounted with two knurled-head screws to PHYTO-PAM-II compact version instruments. Delivery package includes a POM mounting device for light sensor US-SQS/WB.
Dimensions
Ø 49 mm, with connectors and cuvette 64 mm x 74 mm
Weight
150 g
Dimensions

Base plate, 40 cm x 30 cm

Height

73.5 cm, diameter 1.5 cm

Weight

2.8 kg

Sockets
CHARGE socket for Battery Charger MINI-PAM/L, AUX socket for US-SQS/WB Spherical Micro Quantum Sensor, USB socket, socket for Miniature Magnetic Stirrer PHYTO-MS, DETECTOR socket for connecting PHYTO-II-D Photomultiplier-Detector Unit, LED ARRAY and FLUO ML sockets for connecting PHYTO-II-E Multi-Color-Emitter Unit
User interface
Windows computer with PhytoWin-3 software
Communication
USB 2.0 and USB 3.0 compatible
Power supply
Rechargeable sealed lead-acid battery 12 V/2 Ah; Battery Charger MINI-PAM/L (100 to 240 V AC)
Dimensions
29 cm x 30 cm x 18 cm (l x w x h), aluminum housing with carrying handle
Power consumption
Basic operation, 1.5 W; with Measuring and Actinic Light at maximal currents, 4.5 W. Saturation Pulse at maximum intensity, 7 W
Weight
4.2 kg (including battery)
Operating temperature
-5 to +40 °C
Chip-on-board multi-wavelength measuring light LED emitter
440, 480, 540, 590 and 625 nm for pulse-modulated measuring light; 2 intensity settings; 8 settings of pulse frequency and 3 settings of Auto MF-high pulse frequency
Chip-on-board multi-wavelength actinic LED array
440, 480, 540, 590, 625 and 420-640 nm (white) for continuous actinic illumination, up to 3000 µmol m-2 s-1 PAR; actinic pulses for Fast Kinetics, up to 11000 µmol m-2 s-1 PAR; saturation pulses up to 12000 µmol m-2 s-1 PAR
Far-Red LED
Far-Red LED
Dimension
6.6 cm x 10.6 cm x 10 cm (l x w x h)
Weight
490 g
Signal detection
Photomultiplier detector based on Photosensor Module H-10720 (Hamamatsu)
Standard detector filter
long-pass filter > 650 nm
Weight
510 g
Dimension
5.3 cm x 11 cm x 7.1 cm (l x w x h)
Input
100 to 240 V AC, 47 to 63 Hz
Output
19 V DC, 3.7 A
Operating temperature
0 to 40 °C
Dimensions
15 cm x 6 cm x 3 cm (L x W x H)
Weight
300 g

Power-and-Control Unit US-T/DR

Display
Three line LCD display
Control range
0 °C to 50 °C at 0.1 K steps
Operating voltage
11 V - 14 V DC
Maximum Peltier current
1 A
Size
105 mm x 90 mm x 130 mm (W x H x D)
Weight
0.57 kg

Peltier Heat-Transfer Head US-T/DS

Achievable temperatures
12 K below ambient temperature, 15 K above ambient temperature (Quartz cuvette placed in Optical Unit for Suspensions ED-101US/MD with 1.5 mL water and stirrer PHYTO-MS on)
Size
⌀ 55 mm, 110 mm height
Cable length
130 cm
Weight

0.29 kg (including cable)

AC Adapter

Input

100 V - 240 V AC 1.5 A 50-60 Hz

Output
12 V DC 5.5 A
Size

130 mm x 56 mm x 30 mm (L x W x H)

Weight
0.50 kg (including cable)
Temperature Control Block ED-101US/T
Sectioned block with central 10 x 10 mm opening to be mounted on top of the ED-101US/MD unit; to be connected to external flow-through water bath (not included), weight 250 g
Miniature Magnetic Stirrer PHYTO-MS
Based on device manufactured by h+p (type Variomag-Mini); featuring adapter to be mounted in bottom port of the Optical Unit ED-101US/MD; powered and controlled by the Power-and-Control-Unit DKN-C
Spherical Micro Quantum Sensor US-SQS/WB
3.7 mm ø diffusing sphere coupled to integrated PAR sensor via 2 mm diameter fiber; compact amplifier unit and special holder for mounting on Optical Unit ED-101US/MD; to be connected to the Power-and-Control Unit DKN-C
Design

Black-anodized aluminum body with central 10 x 10 mm glass cuvette; for attachment of Measuring Heads and Miniature Magnetic Stirrer PHYTO-MS; additional ports for attachment of two additional measuring heads

Weight

750 g

Temperature Control Block ED-101US/T
Sectioned block with central 10 x 10 mm opening to be mounted on top of the ED-101US/MD unit; to be connected to external flow-through water bath (not included), weight: 250 g
Miniature Magnetic Stirrer PHYTO-MS

Based on a device manufactures by h+p (type Variomag-Mini); featuring adapter to be mounted in the bottom port of the Optical Unit ED-101US/MD; powered and controlled by the Power-and-Control-Unit

Spherical Micro Quantum Sensor US-SQS/WB

3.7 mm Ø diffusing sphere coupled to integrated PAR sensor via 2 mm diameter fiber; compact amplifier unit and special holder for mounting on Optical Unit ED-101US/MD; to be connected tot the Power-and-Control Unit

Design

Aluminium box with custom foam packing for PHYTO-PAM-II and accessories

Dimensions

60 cm x 40 cm x 35 cm (l x w x h)

Weight

5 kg

Design

Aluminium box with custom foam packing for PHYTO-PAM-II and accessories

Dimensions

60 cm x 40 cm x 35 cm (l x w x h)

Weight

6 kg

Application

Suspension cuvette

Connects to

WATER-PAM, DUAL-PAM-100, 
ULM-500 (AUX), PAM-2500 and MINI-PAM (instead of Leaf Clip Holder 2030-B or 2060 M)

Connector

Same as Leaf Clip Holder 2030-B Includes hood for suspension cuvette, amplifier for aux input of PAM Control

Signal output

0…2.5 V DC / 0…1000 μmol m-2 s-1 or 0…2.5 V DC / 0…20.000 μmol m-2 s-1 Power
Provided by connected instrument

Cable length
3 m + 0.5 m
Size

Sensor: Diameter 1 cm
Length: 11 cm
Hood: Diameter 3.4 cm height: 3.2 cm
Amplifier: 5 x 5 x 5 cm (W x L x H)

Weight

175 g

Accessories

Design

Magnetic stirrer driven by a rotating magnetic field; the PHYTO-MS is connected to Power-and-Control Unit PHYTO-II-C; a special adapter plug allows the insertion in the bottom port of the Optical Unit ED-101US/MP

Weight
16 g
Design
Pump interface box. USB- analog 0-10 V converter for PhytoWin-3 controlled operation of a peristaltic pump.
Dimensions
metal housing: 8 cm x 4 cm x 2 cm, cable 100 cm 0- 10V out, USB-device cable: 1.5 m
Weight
380 g
Design
Set of 10 perspex stirring paddle for WATER-II/S
Design
Miniature stirring motor in plastic housing with adapter to mount on top of the PHYTO-PAM-II/ED cuvette; equipped with disposable Perspex stirring paddle; self-contained unit featuring long-life 3 V Lithium Battery; potentiometer for adjustment of stirring rate
Dimensions
8 cm x 5 cm x 3 cm (L x W x H)
Weight
95 g (incl. battery)
Design

Cylindrical housing with 14 mm diameter, height 16 mm. Diffuser diameter: 5 mm, 3 m cable and BNC connector

Sensor
Cosine corrected selective PAR sensor for measuring range 0 to 20000 μmol m-2 s-1. Shipped together with the Adapter Set MQS-B/A for the calibration of the internal light list of the PHYTO-PAM FIBER version and WATER-PAM FIBER version. The sensor part (MQS-B) can be directly connected to the Universal Light Meter ULM-500

PhytoWin-3 Software

PHYTO-PAM-II Operation Software

General Features and Graphical User Interface
PhytoWin-3 is the dedicated control and data analysis software for PHYTO-PAM-II instruments. Designed to run on Windows PCs, it provides full operational control of the instrument and supports the setup, execution, and analysis of various fluorescence measurement protocols.

The nine software tabs are:

Channels: Raw data, fluorescence data at 5 different excitation wavelengths

Settings: Control tab for settings such as measuring pulse frequency, actinic intensity, saturation pulse width and intensity, clock interval, etc.

Algae: Deconvoluted fluorescence data for cyanobacteria, green algae, diatoms/dinoflagellates and phycoerythrin-containing organisms (e.g. cryptophytes) based on reference excitation spectra; user interface for Chl a determination

Light Curve: Graphic display of light response curves; effective quantum yield and relative electron transport rate (ETR) as a function of PAR, Curve fitting based on a modified Eilers & Peeters (1988) or Platt (1980) model

Slow Kinetics: Graphic display of slow kinetics recorded in the SP-analysis mode

Report: Shows all the measured data and instrument settings stored, export of data for other programs

Reference: Recording and display of reference excitation spectra of cyanobacteria, green algae, diatoms/dinoflagellates and phycoerythrin-containing organisms

Fluo Spec: Display of measuring light characteristics and non-normalized reference excitation spectra

Fast Kinetics: Control and graphical display of fast kinetics analysis providing information on the wavelength-dependent absorption cross-section of PS II, σII(λ)

Free software updates will keep your instrument always up-to date on latest developments.
PhytoWin-3 cannot be used to operate first generation PHYTO-PAM instruments.

PhytoWin Software

Operation Software for the FIBER Version of the PHYTO-PAM

FIBER Version instruments belong to the first generation PHYTO-PAM, which are controlled by PhytoWin software on a Windows PC. Please note that these four-wavelength PHYTO-PAM models are not compatible with PhytoWin-3 software.

PhytoWin provides a user-friendly interface with seven dedicated tabs—Channels, Algae, Report, Light Curve, Settings, Reference, and Delta F—that support both the visualization and analysis of raw and processed fluorescence data. These modules allow for a comprehensive evaluation of photosynthetic parameters and phytoplankton group composition of up to three algal groups.