DIVING-PAM-II

Product

Underwater Fluorometer with Miniature Spectrometer

WinControl-3 Software

General Features and Graphical User Interface

The DIVING-PAM-II can be operated by Windows computers running the WinControl-3 software. The same software operates the fluorometers MICRO-PAM, MINI-PAM-II, MONITORING-PAM, and JUNIOR-PAM, PAM fluorometers operated via the PAM-CONTROL interface (MICROSCOPY-PAM, MICROFIBER-PAM and WATER-PAM) as well as the Universal Light Meter ULM-500.
Saturation pulse analysis and automated experimental protocols can be performed under control of the software WinControl-3 but also by the DIVING-PAM-II internal software which is active when the DIVING-PAM-II is operated autonomously. In the latter case, experimental parameters are entered using waterproof infrared reflection switches. Continuous recording of fluorescence and modeling of light curves, however, requires WinControl-3.

Induction Curve Window: The window displays continuous PAM fluorescence (Ft) as black line. The non-photochemical quenching parameter, NPQ, is drawn in blue and electron transport rate (ETR) is shown in red.
Induction Curve Window: The window displays continuous PAM fluorescence (Ft) as black line. The non-photochemical quenching parameter, NPQ, is drawn in blue and electron transport rate (ETR) is shown in red.

Data Evaluation

Saturating pulse analysis with automatic detection and calculation of standard fluorescence parameters: F0, FM, F0’ (measured or calculated), FM’, FV/FM, qP, qL, qN, NPQ, Y(II), Y(NPQ), Y(NO), ETR.

Data Export

Export in Excel, CSV or TXT format of original fluorescence traces, saturating pulse analysis data and parameter estimates of light response curves.

Automated Routines

Repetitive triggering of many fluorometer functions (e.g., saturation pulse analysis, induction curves). Automatic execution of short-term illumination, light and induction curves, offset correction and calibration of internal light sensor.

Customer-defined Measuring Protocols

Execution of customized experimental procedures using batch files.